The myeloperoxidase gene proximal enhancer directs hematopoietic specific expression in transgenic mice

S. A. Lira, A. D. Friedman

Research output: Contribution to journalArticle

Abstract

The myeloperoxidase (MPO) gene is expressed specifically in immature myeloid cells. The MPO gene includes a promoter proximal enhancer which is coincident with DNaseI hypersensitive chromatin sites and is specifically active in myeloid cell lines. We developed transgenic murine lines in which 1.3 kb of murine MPO proximal 5' flanking region DNA was linked to a TATAA homology and RNA initiation site derived from the HSV-TK promoter and to a luciferase reporter (MPOTKLUC). In each of six founder lines, high-level luciferase activity was evident in marrow, thymus and spleen. Modest- to high-level luciferase expression was also evident in brain and in the heart in several of the lines, and luciferase activity was at or near background levels in lung, liver, kidney, stomach, colon, bladder, skeletal muscle, skin and small intestine in all of the MPOTKLUC transgenic mice. Within marrow cells, luciferase activity was evident in myeloid (GR-1+), B lymphoid (B220 +) and T-lymphoid (CD4+) cells. Additional regulatory regions, thus, may be required to further restrict MPO gene expression to immature myeloid cells.

Original languageEnglish (US)
Pages (from-to)311-314
Number of pages4
JournalGene
Volume197
Issue number1-2
DOIs
StatePublished - Sep 15 1997

Keywords

  • Differentiation
  • Gene expression
  • Granulocyte
  • Hematopoiesis

ASJC Scopus subject areas

  • Genetics

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