The murine myeloperoxidase gene contains a bipartite distal enhancer, including a novel region regulated by PEBP2/CBF

Alan D. Friedman; Martin Britos-Bray; Joseph Suzow

doi:10.1016/S0145-2126(96)00035-5

The murine myeloperoxidase gene contains a bipartite distal enhancer, including a novel region regulated by PEBP2/CBF

Alan D. Friedman, Martin Britos-Bray, Joseph Suzow

School of Medicine

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The myeloperoxidase (MPO) gene is expressed specifically in immature myeloid cells. DNA (27 kb) derived from the murine MPO gene was subcloned into nine segments, and each examined for its ability to enhance transcription in 32D cl3 myeloid cells. Only one segment, besides the known proximal enhancer, was active. Deletional analysis of this region delimited two functional regions. One is located 3.4 kb upstream of the proximal enhancer and was also recently identified in a different cell system. The second novel region is a 133 bp segment located 1.1 kb further upstream. This region, like the proximal enhancer, binds and is regulated by PEBP2/CBF. The PEBP21CBF binding-site in the distal enhancer, AACCAAT, differs from the PEBP2/CBF consensus, PuACCPuCA. Copyright (C) 1996 Elsevier Science Ltd.

Original language	English (US)
Pages (from-to)	809-815
Number of pages	7
Journal	Leukemia Research
Volume	20
Issue number	10
DOIs	https://doi.org/10.1016/S0145-2126(96)00035-5
State	Published - Oct 1996

Keywords

Differentiation
Enhancer
Leukemia
Myeloperoxidase
Myelopoiesis
PEBP2/CB F

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.1016/S0145-2126(96)00035-5

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title = "The murine myeloperoxidase gene contains a bipartite distal enhancer, including a novel region regulated by PEBP2/CBF",

abstract = "The myeloperoxidase (MPO) gene is expressed specifically in immature myeloid cells. DNA (27 kb) derived from the murine MPO gene was subcloned into nine segments, and each examined for its ability to enhance transcription in 32D cl3 myeloid cells. Only one segment, besides the known proximal enhancer, was active. Deletional analysis of this region delimited two functional regions. One is located 3.4 kb upstream of the proximal enhancer and was also recently identified in a different cell system. The second novel region is a 133 bp segment located 1.1 kb further upstream. This region, like the proximal enhancer, binds and is regulated by PEBP2/CBF. The PEBP21CBF binding-site in the distal enhancer, AACCAAT, differs from the PEBP2/CBF consensus, PuACCPuCA. Copyright (C) 1996 Elsevier Science Ltd.",

keywords = "Differentiation, Enhancer, Leukemia, Myeloperoxidase, Myelopoiesis, PEBP2/CB F",

author = "Friedman, {Alan D.} and Martin Britos-Bray and Joseph Suzow",

note = "Funding Information: Acknowledgements-The authors thank G. Rovera for the murine MPO proximal 5{\textquoteright} flanking region DNA, T. Lanahan for the Balb/c genomic DNA library, and N. Speck for the CBFfl antisera.T his work was supported by the Searle Scholars Program and the NIH (HL51388).",

year = "1996",

month = oct,

doi = "10.1016/S0145-2126(96)00035-5",

language = "English (US)",

volume = "20",

pages = "809--815",

journal = "Leukemia Research",

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T1 - The murine myeloperoxidase gene contains a bipartite distal enhancer, including a novel region regulated by PEBP2/CBF

AU - Friedman, Alan D.

AU - Britos-Bray, Martin

AU - Suzow, Joseph

N1 - Funding Information: Acknowledgements-The authors thank G. Rovera for the murine MPO proximal 5’ flanking region DNA, T. Lanahan for the Balb/c genomic DNA library, and N. Speck for the CBFfl antisera.T his work was supported by the Searle Scholars Program and the NIH (HL51388).

PY - 1996/10

Y1 - 1996/10

N2 - The myeloperoxidase (MPO) gene is expressed specifically in immature myeloid cells. DNA (27 kb) derived from the murine MPO gene was subcloned into nine segments, and each examined for its ability to enhance transcription in 32D cl3 myeloid cells. Only one segment, besides the known proximal enhancer, was active. Deletional analysis of this region delimited two functional regions. One is located 3.4 kb upstream of the proximal enhancer and was also recently identified in a different cell system. The second novel region is a 133 bp segment located 1.1 kb further upstream. This region, like the proximal enhancer, binds and is regulated by PEBP2/CBF. The PEBP21CBF binding-site in the distal enhancer, AACCAAT, differs from the PEBP2/CBF consensus, PuACCPuCA. Copyright (C) 1996 Elsevier Science Ltd.

AB - The myeloperoxidase (MPO) gene is expressed specifically in immature myeloid cells. DNA (27 kb) derived from the murine MPO gene was subcloned into nine segments, and each examined for its ability to enhance transcription in 32D cl3 myeloid cells. Only one segment, besides the known proximal enhancer, was active. Deletional analysis of this region delimited two functional regions. One is located 3.4 kb upstream of the proximal enhancer and was also recently identified in a different cell system. The second novel region is a 133 bp segment located 1.1 kb further upstream. This region, like the proximal enhancer, binds and is regulated by PEBP2/CBF. The PEBP21CBF binding-site in the distal enhancer, AACCAAT, differs from the PEBP2/CBF consensus, PuACCPuCA. Copyright (C) 1996 Elsevier Science Ltd.

KW - Differentiation

KW - Enhancer

KW - Leukemia

KW - Myeloperoxidase

KW - Myelopoiesis

KW - PEBP2/CB F

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AN - SCOPUS:0030271491

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ER -

The murine myeloperoxidase gene contains a bipartite distal enhancer, including a novel region regulated by PEBP2/CBF

Abstract

Keywords

ASJC Scopus subject areas

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