The murine myeloperoxidase gene contains a bipartite distal enhancer, including a novel region regulated by PEBP2/CBF

Alan D. Friedman, Martin Britos-Bray, Joseph Suzow

Research output: Contribution to journalArticlepeer-review

Abstract

The myeloperoxidase (MPO) gene is expressed specifically in immature myeloid cells. DNA (27 kb) derived from the murine MPO gene was subcloned into nine segments, and each examined for its ability to enhance transcription in 32D cl3 myeloid cells. Only one segment, besides the known proximal enhancer, was active. Deletional analysis of this region delimited two functional regions. One is located 3.4 kb upstream of the proximal enhancer and was also recently identified in a different cell system. The second novel region is a 133 bp segment located 1.1 kb further upstream. This region, like the proximal enhancer, binds and is regulated by PEBP2/CBF. The PEBP21CBF binding-site in the distal enhancer, AACCAAT, differs from the PEBP2/CBF consensus, PuACCPuCA. Copyright (C) 1996 Elsevier Science Ltd.

Original languageEnglish (US)
Pages (from-to)809-815
Number of pages7
JournalLeukemia Research
Volume20
Issue number10
DOIs
StatePublished - Oct 1996

Keywords

  • Differentiation
  • Enhancer
  • Leukemia
  • Myeloperoxidase
  • Myelopoiesis
  • PEBP2/CB F

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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