The mouse viral outgrowth assay: Avatars for the detection of HIV-1 reservoirs

Kelly A. Metcalf Pate; Joel N. Blankson

doi:10.1186/s12977-017-0376-z

The mouse viral outgrowth assay: Avatars for the detection of HIV-1 reservoirs

Kelly A. Metcalf Pate, Joel N. Blankson

School of Medicine

Research output: Contribution to journal › Review article › peer-review

6 Scopus citations

Abstract

Sensitive assays are needed for the detection of residual viral reservoirs in HIV-1-infected subjects on suppressive combination antiretroviral therapy regimens to determine whether eradication strategies are effective. Mouse viral outgrowth assays have recently been developed and have the potential to be more sensitive than traditional in vitro quantitative viral outgrowth assays. In this article we describe these assays and review several studies that have used them to measure the latent reservoir.

Original language	English (US)
Article number	52
Journal	Retrovirology
Volume	14
Issue number	1
DOIs	https://doi.org/10.1186/s12977-017-0376-z
State	Published - Nov 21 2017

Keywords

HIV-1
Latency
Mouse viral outgrowth assay
QVOA
Reservoir
SIV

ASJC Scopus subject areas

Virology
Infectious Diseases

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10.1186/s12977-017-0376-z

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abstract = "Sensitive assays are needed for the detection of residual viral reservoirs in HIV-1-infected subjects on suppressive combination antiretroviral therapy regimens to determine whether eradication strategies are effective. Mouse viral outgrowth assays have recently been developed and have the potential to be more sensitive than traditional in vitro quantitative viral outgrowth assays. In this article we describe these assays and review several studies that have used them to measure the latent reservoir.",

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AB - Sensitive assays are needed for the detection of residual viral reservoirs in HIV-1-infected subjects on suppressive combination antiretroviral therapy regimens to determine whether eradication strategies are effective. Mouse viral outgrowth assays have recently been developed and have the potential to be more sensitive than traditional in vitro quantitative viral outgrowth assays. In this article we describe these assays and review several studies that have used them to measure the latent reservoir.

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KW - Latency

KW - Mouse viral outgrowth assay

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KW - Reservoir

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The mouse viral outgrowth assay: Avatars for the detection of HIV-1 reservoirs

Abstract

Keywords

ASJC Scopus subject areas

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