The mouse ribosomal DNA promoter has more stringent requirements in vivo than in vitro

Sheryl L. Henderson, Barbara Sollner-Webb

Research output: Contribution to journalArticle

Abstract

Using mouse ribosomal DNA templates bearing polymerase I terminators to prevent transcriptional interference (S. L. Henderson, K. Ryan, and B. Sollner-Webb, Genes Dev. 3:212-223, 1989) and facilitate promoter analysis in intact cells, we demonstrate that a -140 promoter domain (as well as the core region) is essential for appreciable levels of initiation in vivo. This in vivo polymerase I promoter can also be detected in vitro but only under very stringent conditions.

Original languageEnglish (US)
Pages (from-to)4970-4973
Number of pages4
JournalMolecular and cellular biology
Volume10
Issue number9
DOIs
StatePublished - Sep 1990

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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