TY - JOUR
T1 - The molecular weight and oligomerization of rabbit thrombomodulin as assessed by sedimentation equilibrium
AU - Winnard, Paul T.
AU - Esmon, Charles T.
AU - Laue, Thomas M.
N1 - Funding Information:
r This work is supported by Grants-in-Aid 85-0781 and 8’7-1092 from the American Heart Association, Inc., National Institutes of Health R01 HL30430, and National Science Foundation BBS 81815. This is scientific contribution No. 1571 from the New Hampshire Agricultural Experiment Station. The authors thank Theresa Ridgeway and Daryl Lyons for their assistance in reading and analyzing the interferograms, and Glen Pipher and Barbara Carpenter for assistance in the TM isolation. ‘To whom correspondence should be addressed.
PY - 1989/2/15
Y1 - 1989/2/15
N2 - Lubrol-solubilized rabbit thrombomodulin has been examined by equilibrium sedimentation in buffers that include sufficient D2O to make the detergent neutrally buoyant. Data were acquired at rotor speeds from 12,000 to 28,000 rpm from two thrombomodulin preparations, at protein concentrations from 0.01 to 0.07%, and in buffer containing 0.01 to 0.23% Lubrol. Examination of the data from different rotor speeds shows that the thrombomodulin exists as a heterogeneous mixture containing monomer (Mr 65,000), trimer, and higher oligomers. The oligomers do not equilibrate over the time scale of the experiment. The weight fraction as monomer varies from preparation to preparation, and appears to be independent of detergent concentration. Thus, experimenters should be cautious when interpreting binding or kinetic results obtained under similar buffer conditions.
AB - Lubrol-solubilized rabbit thrombomodulin has been examined by equilibrium sedimentation in buffers that include sufficient D2O to make the detergent neutrally buoyant. Data were acquired at rotor speeds from 12,000 to 28,000 rpm from two thrombomodulin preparations, at protein concentrations from 0.01 to 0.07%, and in buffer containing 0.01 to 0.23% Lubrol. Examination of the data from different rotor speeds shows that the thrombomodulin exists as a heterogeneous mixture containing monomer (Mr 65,000), trimer, and higher oligomers. The oligomers do not equilibrate over the time scale of the experiment. The weight fraction as monomer varies from preparation to preparation, and appears to be independent of detergent concentration. Thus, experimenters should be cautious when interpreting binding or kinetic results obtained under similar buffer conditions.
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U2 - 10.1016/0003-9861(89)90115-X
DO - 10.1016/0003-9861(89)90115-X
M3 - Article
C2 - 2537060
AN - SCOPUS:0024520191
SN - 0003-9861
VL - 269
SP - 339
EP - 344
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -