The molecular systemic and local effects of intratendinous injection of platelet rich plasma in tendinosis: Preliminary results on a rat model with ELISA method

Benjamin Dallaudiere, Liliane Louedec, Marie Paule Jacob Lenet, Lionel Pesquer, Elvind Blaise, Anne Perozziello, Jean Baptiste Michel, Maryse Moinard, Philippe Meyer, Jean Michel Serfaty

Research output: Contribution to journalArticle

Abstract

Purpose: the aim of our study was thus to quantify the effect of Platelet Rich Plasma (PRP) injection on systemic and local growth factors and to identify molecular markers in a rat model of patellar and Achilles tendinosis treated with PRP. Material and method: twenty two rats were used for the study. Two healthy rats were used as control (T-). We induced tendinosis (T+) in 20 rats (80 tendons by injecting under ultrasonography (US) guidance Collagenase 1® (day 0 = D0, patellar=40 and Achilles=40). At D3, these 20 rats with tendinosis were separated in treatment by either PRP (PRPT+, n=28), physiological serum (PST+, n=28, control) USguided intratendinous injection, or without no PRP or PS (T+, n=24, control of natural evolution of tendinopathy). Follow-up at D7, D13, D18 and D25 using serum sample and local tendon removal with ELISA technics and comparison between the 3 groups were performed. Results: during biological follow up, comparison of all serum samples of PRPT+, PST+ and T+ groups showed no significant modification of their biological markers at D7, D13, D18 and D25 (p>0.22). Comparison of immunological sample tendon markers of PRPT+, PST+ and T+ groups also showed no significant modification of markers at D7, D13, D18 and D25 (p>0.16) considering each biological marker and also all subgroups confounded. Conclusion: our study strongly suggests that a single intratendinous US-guided injection of PRP in Achilles and patellar T+ doesn’t increase biological markers such as growth factors compared to a control group in mid-term and long-term follow-up.

Original languageEnglish (US)
Pages (from-to)99-105
Number of pages7
JournalMuscles, Ligaments and Tendons Journal
Volume5
Issue number2
DOIs
StatePublished - Jul 10 2015
Externally publishedYes

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Tendinopathy
Platelet-Rich Plasma
Enzyme-Linked Immunosorbent Assay
Injections
Tendons
Biomarkers
Ultrasonography
Intercellular Signaling Peptides and Proteins
Serum
Control Groups

Keywords

  • ELISA
  • Platelet
  • PRP
  • Rat
  • Tendinosis

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

The molecular systemic and local effects of intratendinous injection of platelet rich plasma in tendinosis : Preliminary results on a rat model with ELISA method. / Dallaudiere, Benjamin; Louedec, Liliane; Lenet, Marie Paule Jacob; Pesquer, Lionel; Blaise, Elvind; Perozziello, Anne; Michel, Jean Baptiste; Moinard, Maryse; Meyer, Philippe; Serfaty, Jean Michel.

In: Muscles, Ligaments and Tendons Journal, Vol. 5, No. 2, 10.07.2015, p. 99-105.

Research output: Contribution to journalArticle

Dallaudiere, B, Louedec, L, Lenet, MPJ, Pesquer, L, Blaise, E, Perozziello, A, Michel, JB, Moinard, M, Meyer, P & Serfaty, JM 2015, 'The molecular systemic and local effects of intratendinous injection of platelet rich plasma in tendinosis: Preliminary results on a rat model with ELISA method', Muscles, Ligaments and Tendons Journal, vol. 5, no. 2, pp. 99-105. https://doi.org/10.11138/mltj/2015.5.2.099
Dallaudiere, Benjamin ; Louedec, Liliane ; Lenet, Marie Paule Jacob ; Pesquer, Lionel ; Blaise, Elvind ; Perozziello, Anne ; Michel, Jean Baptiste ; Moinard, Maryse ; Meyer, Philippe ; Serfaty, Jean Michel. / The molecular systemic and local effects of intratendinous injection of platelet rich plasma in tendinosis : Preliminary results on a rat model with ELISA method. In: Muscles, Ligaments and Tendons Journal. 2015 ; Vol. 5, No. 2. pp. 99-105.
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abstract = "Purpose: the aim of our study was thus to quantify the effect of Platelet Rich Plasma (PRP) injection on systemic and local growth factors and to identify molecular markers in a rat model of patellar and Achilles tendinosis treated with PRP. Material and method: twenty two rats were used for the study. Two healthy rats were used as control (T-). We induced tendinosis (T+) in 20 rats (80 tendons by injecting under ultrasonography (US) guidance Collagenase 1{\circledR} (day 0 = D0, patellar=40 and Achilles=40). At D3, these 20 rats with tendinosis were separated in treatment by either PRP (PRPT+, n=28), physiological serum (PST+, n=28, control) USguided intratendinous injection, or without no PRP or PS (T+, n=24, control of natural evolution of tendinopathy). Follow-up at D7, D13, D18 and D25 using serum sample and local tendon removal with ELISA technics and comparison between the 3 groups were performed. Results: during biological follow up, comparison of all serum samples of PRPT+, PST+ and T+ groups showed no significant modification of their biological markers at D7, D13, D18 and D25 (p>0.22). Comparison of immunological sample tendon markers of PRPT+, PST+ and T+ groups also showed no significant modification of markers at D7, D13, D18 and D25 (p>0.16) considering each biological marker and also all subgroups confounded. Conclusion: our study strongly suggests that a single intratendinous US-guided injection of PRP in Achilles and patellar T+ doesn’t increase biological markers such as growth factors compared to a control group in mid-term and long-term follow-up.",
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AU - Louedec, Liliane

AU - Lenet, Marie Paule Jacob

AU - Pesquer, Lionel

AU - Blaise, Elvind

AU - Perozziello, Anne

AU - Michel, Jean Baptiste

AU - Moinard, Maryse

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AU - Serfaty, Jean Michel

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