The molecular mechanism underlying Roberts syndrome involves loss of ESCO2 acetyltransferase activity

Miriam Gordillo, Hugo Vega, Alison H. Trainer, Fajian Hou, Norio Sakai, Ricardo Luque, Hülya Kayserili, Seher Basaran, Flemming Skovby, Raoul C M Hennekam, Maria L Giovannucci Uzielli, Rhonda E. Schnur, Sylvie Manouvrier, Susan Chang, Edward Blair, Jane A. Hurst, Francesca Forzano, Moritz Meins, Kalle O J Simola, Annick Raas-rothschildRoger A. Schultz, Lisa D. Mcdaniel, Keiichi Ozono, Koji Inui, Hui Zou, Ethylin Wang Jabs

Research output: Contribution to journalArticle

Abstract

Roberts syndrome/SC phocomelia (RBS) is an autosomal recessive disorder with growth retardation, craniofacial abnormalities and limb reduction. Cellular alterations in RBS include lack of cohesion at the heterochromatic regions around centromeres and the long arm of the Y chromosome, reduced growth capacity, and hypersensitivity to DNA damaging agents. RBS is caused by mutations in ESCO2, which encodes a protein belonging to the highly conserved Eco1/Ctf7 family of acetyltransferases that is involved in regulating sister chromatid cohesion. We identified 10 new mutations expanding the number to 26 known ESCO2 mutations. We observed that these mutations result in complete or partial loss of the acetyltransferase domain except for the only missense mutation that occurs in this domain (c.1615T>G, W539G). To investigate the mechanism underlying RBS, we analyzed ESCO2 mutations for their effect on enzymatic activity and cellular phenotype. We found that ESCO2 W539G results in loss of autoacetyltransferase activity. The cellular phenotype produced by this mutation causes cohesion defects, proliferation capacity reduction and mitomycin C sensitivity equivalent to those produced by frameshift and nonsense mutations associated with decreased levels of mRNA and absence of protein. We found decreased proliferation capacity in RBS cell lines associated with cell death, but not with increased cell cycle duration, which could be a factor in the development of phocomelia and cleft palate in RBS. In summary, we provide the first evidence that loss of acetyltransferase activity contributes to the pathogenesis of RBS, underscoring the essential role of the enzymatic activity of the Eco1p family of proteins.

Original languageEnglish (US)
Pages (from-to)2172-2180
Number of pages9
JournalHuman Molecular Genetics
Volume17
Issue number14
DOIs
StatePublished - Jul 2008

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Ectromelia
Acetyltransferases
Mutation
Craniofacial Abnormalities
Growth Disorders
Phenotype
Frameshift Mutation
Proteins
Chromatids
Roberts Syndrome
Centromere
Nonsense Codon
Y Chromosome
Cleft Palate
Mitomycin
Missense Mutation
Cell Cycle
Hypersensitivity
Cell Death
Extremities

ASJC Scopus subject areas

  • Genetics

Cite this

Gordillo, M., Vega, H., Trainer, A. H., Hou, F., Sakai, N., Luque, R., ... Jabs, E. W. (2008). The molecular mechanism underlying Roberts syndrome involves loss of ESCO2 acetyltransferase activity. Human Molecular Genetics, 17(14), 2172-2180. https://doi.org/10.1093/hmg/ddn116

The molecular mechanism underlying Roberts syndrome involves loss of ESCO2 acetyltransferase activity. / Gordillo, Miriam; Vega, Hugo; Trainer, Alison H.; Hou, Fajian; Sakai, Norio; Luque, Ricardo; Kayserili, Hülya; Basaran, Seher; Skovby, Flemming; Hennekam, Raoul C M; Uzielli, Maria L Giovannucci; Schnur, Rhonda E.; Manouvrier, Sylvie; Chang, Susan; Blair, Edward; Hurst, Jane A.; Forzano, Francesca; Meins, Moritz; Simola, Kalle O J; Raas-rothschild, Annick; Schultz, Roger A.; Mcdaniel, Lisa D.; Ozono, Keiichi; Inui, Koji; Zou, Hui; Jabs, Ethylin Wang.

In: Human Molecular Genetics, Vol. 17, No. 14, 07.2008, p. 2172-2180.

Research output: Contribution to journalArticle

Gordillo, M, Vega, H, Trainer, AH, Hou, F, Sakai, N, Luque, R, Kayserili, H, Basaran, S, Skovby, F, Hennekam, RCM, Uzielli, MLG, Schnur, RE, Manouvrier, S, Chang, S, Blair, E, Hurst, JA, Forzano, F, Meins, M, Simola, KOJ, Raas-rothschild, A, Schultz, RA, Mcdaniel, LD, Ozono, K, Inui, K, Zou, H & Jabs, EW 2008, 'The molecular mechanism underlying Roberts syndrome involves loss of ESCO2 acetyltransferase activity', Human Molecular Genetics, vol. 17, no. 14, pp. 2172-2180. https://doi.org/10.1093/hmg/ddn116
Gordillo, Miriam ; Vega, Hugo ; Trainer, Alison H. ; Hou, Fajian ; Sakai, Norio ; Luque, Ricardo ; Kayserili, Hülya ; Basaran, Seher ; Skovby, Flemming ; Hennekam, Raoul C M ; Uzielli, Maria L Giovannucci ; Schnur, Rhonda E. ; Manouvrier, Sylvie ; Chang, Susan ; Blair, Edward ; Hurst, Jane A. ; Forzano, Francesca ; Meins, Moritz ; Simola, Kalle O J ; Raas-rothschild, Annick ; Schultz, Roger A. ; Mcdaniel, Lisa D. ; Ozono, Keiichi ; Inui, Koji ; Zou, Hui ; Jabs, Ethylin Wang. / The molecular mechanism underlying Roberts syndrome involves loss of ESCO2 acetyltransferase activity. In: Human Molecular Genetics. 2008 ; Vol. 17, No. 14. pp. 2172-2180.
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abstract = "Roberts syndrome/SC phocomelia (RBS) is an autosomal recessive disorder with growth retardation, craniofacial abnormalities and limb reduction. Cellular alterations in RBS include lack of cohesion at the heterochromatic regions around centromeres and the long arm of the Y chromosome, reduced growth capacity, and hypersensitivity to DNA damaging agents. RBS is caused by mutations in ESCO2, which encodes a protein belonging to the highly conserved Eco1/Ctf7 family of acetyltransferases that is involved in regulating sister chromatid cohesion. We identified 10 new mutations expanding the number to 26 known ESCO2 mutations. We observed that these mutations result in complete or partial loss of the acetyltransferase domain except for the only missense mutation that occurs in this domain (c.1615T>G, W539G). To investigate the mechanism underlying RBS, we analyzed ESCO2 mutations for their effect on enzymatic activity and cellular phenotype. We found that ESCO2 W539G results in loss of autoacetyltransferase activity. The cellular phenotype produced by this mutation causes cohesion defects, proliferation capacity reduction and mitomycin C sensitivity equivalent to those produced by frameshift and nonsense mutations associated with decreased levels of mRNA and absence of protein. We found decreased proliferation capacity in RBS cell lines associated with cell death, but not with increased cell cycle duration, which could be a factor in the development of phocomelia and cleft palate in RBS. In summary, we provide the first evidence that loss of acetyltransferase activity contributes to the pathogenesis of RBS, underscoring the essential role of the enzymatic activity of the Eco1p family of proteins.",
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AU - Gordillo, Miriam

AU - Vega, Hugo

AU - Trainer, Alison H.

AU - Hou, Fajian

AU - Sakai, Norio

AU - Luque, Ricardo

AU - Kayserili, Hülya

AU - Basaran, Seher

AU - Skovby, Flemming

AU - Hennekam, Raoul C M

AU - Uzielli, Maria L Giovannucci

AU - Schnur, Rhonda E.

AU - Manouvrier, Sylvie

AU - Chang, Susan

AU - Blair, Edward

AU - Hurst, Jane A.

AU - Forzano, Francesca

AU - Meins, Moritz

AU - Simola, Kalle O J

AU - Raas-rothschild, Annick

AU - Schultz, Roger A.

AU - Mcdaniel, Lisa D.

AU - Ozono, Keiichi

AU - Inui, Koji

AU - Zou, Hui

AU - Jabs, Ethylin Wang

PY - 2008/7

Y1 - 2008/7

N2 - Roberts syndrome/SC phocomelia (RBS) is an autosomal recessive disorder with growth retardation, craniofacial abnormalities and limb reduction. Cellular alterations in RBS include lack of cohesion at the heterochromatic regions around centromeres and the long arm of the Y chromosome, reduced growth capacity, and hypersensitivity to DNA damaging agents. RBS is caused by mutations in ESCO2, which encodes a protein belonging to the highly conserved Eco1/Ctf7 family of acetyltransferases that is involved in regulating sister chromatid cohesion. We identified 10 new mutations expanding the number to 26 known ESCO2 mutations. We observed that these mutations result in complete or partial loss of the acetyltransferase domain except for the only missense mutation that occurs in this domain (c.1615T>G, W539G). To investigate the mechanism underlying RBS, we analyzed ESCO2 mutations for their effect on enzymatic activity and cellular phenotype. We found that ESCO2 W539G results in loss of autoacetyltransferase activity. The cellular phenotype produced by this mutation causes cohesion defects, proliferation capacity reduction and mitomycin C sensitivity equivalent to those produced by frameshift and nonsense mutations associated with decreased levels of mRNA and absence of protein. We found decreased proliferation capacity in RBS cell lines associated with cell death, but not with increased cell cycle duration, which could be a factor in the development of phocomelia and cleft palate in RBS. In summary, we provide the first evidence that loss of acetyltransferase activity contributes to the pathogenesis of RBS, underscoring the essential role of the enzymatic activity of the Eco1p family of proteins.

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