The molecular basis of human 3-methylcrotonyl-CoA carboxylase deficiency

M. R. Baumgartner, S. Almashanu, T. Suormala, C. Obie, R. N. Cole, S. Packman, E. R. Baumgartner, D. Valle

Research output: Contribution to journalArticle

Abstract

Isolated biotin-resistant 3-methylcrotonyl-CoA carboxylase (MCC) deficiency is an autosomal recessive disorder of leucine catabolism that appears to be the most frequent organic aciduria detected in tandem mass spectrometry-based neonatal screening programs. The phenotype is variable, ranging from neonatal onset with severe neurological involvement to asymptomatic adults. MCC is a heteromeric mitochondrial enzyme composed of biotin-containing α subunits and smaller β subunits. Here, we report cloning of MCCA and MCCB cDNAs and the organization of their structural genes. We show that a series of 14 MCC-deficient probands defines two complementation groups, CG1 and 2, resulting from mutations in MCCB and MCCA, respectively. We identify five MCCA and nine MCCB mutant alleles and show that missense mutations in each result in loss of function.

Original languageEnglish (US)
Pages (from-to)495-504
Number of pages10
JournalJournal of Clinical Investigation
Volume107
Issue number4
DOIs
StatePublished - Jan 1 2001

ASJC Scopus subject areas

  • Medicine(all)

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    Baumgartner, M. R., Almashanu, S., Suormala, T., Obie, C., Cole, R. N., Packman, S., Baumgartner, E. R., & Valle, D. (2001). The molecular basis of human 3-methylcrotonyl-CoA carboxylase deficiency. Journal of Clinical Investigation, 107(4), 495-504. https://doi.org/10.1172/JCI11948