The mitotic feedback control gene MAD2 encodes the α-subunit of a prenyltransferase

Rong Li, C. Havel, J. A. Watson, A. W. Murray

Research output: Contribution to journalArticle

Abstract

THE mad2-1 mutation inactivates the cell-cycle feedback control that prevents budding yeast cells from leaving mitosis until spindle assembly is complete. The gene product of MAD2 shows significant sequence similarity to the α-subunit of prenyltransferases. Here we isolate a new temperature- sensitive mad2 mutant, mad2-2(tx), and find that MAD2p is required for the membrane association of Ypt1p and Sec4p, two prenylated small GTP-binding proteins involved in protein trafficking. Extracts from mad2-2(tx) mutant cells fail to geranylgeranylate a number of substrates at the non-permissive temperature. mad2-2(tx) is synthetically lethal with bet2-1, a mutation in the gene that encodes for the β-subunit of the Ypt1p and Sec4p geranylgeranyl transferase. Therefore MAD2 and BET2 gene products may physically interact to form a geranylgeranyl transferase complex. In addition, the difference between the phenotypes of mad2-1 and mad2-2(tx) suggests that MAD2 has distinct roles in protein transport and the mitotic feedback control.

Original languageEnglish (US)
Pages (from-to)82-84
Number of pages3
JournalNature
Volume366
Issue number6450
DOIs
StatePublished - 1993
Externally publishedYes

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Dimethylallyltranstransferase
Protein Transport
Transferases
Genes
Mutation
Saccharomycetales
Temperature
Cell Cycle Checkpoints
GTP-Binding Proteins
Mitosis
Phenotype
Membranes

ASJC Scopus subject areas

  • General

Cite this

The mitotic feedback control gene MAD2 encodes the α-subunit of a prenyltransferase. / Li, Rong; Havel, C.; Watson, J. A.; Murray, A. W.

In: Nature, Vol. 366, No. 6450, 1993, p. 82-84.

Research output: Contribution to journalArticle

Li, Rong ; Havel, C. ; Watson, J. A. ; Murray, A. W. / The mitotic feedback control gene MAD2 encodes the α-subunit of a prenyltransferase. In: Nature. 1993 ; Vol. 366, No. 6450. pp. 82-84.
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abstract = "THE mad2-1 mutation inactivates the cell-cycle feedback control that prevents budding yeast cells from leaving mitosis until spindle assembly is complete. The gene product of MAD2 shows significant sequence similarity to the α-subunit of prenyltransferases. Here we isolate a new temperature- sensitive mad2 mutant, mad2-2(tx), and find that MAD2p is required for the membrane association of Ypt1p and Sec4p, two prenylated small GTP-binding proteins involved in protein trafficking. Extracts from mad2-2(tx) mutant cells fail to geranylgeranylate a number of substrates at the non-permissive temperature. mad2-2(tx) is synthetically lethal with bet2-1, a mutation in the gene that encodes for the β-subunit of the Ypt1p and Sec4p geranylgeranyl transferase. Therefore MAD2 and BET2 gene products may physically interact to form a geranylgeranyl transferase complex. In addition, the difference between the phenotypes of mad2-1 and mad2-2(tx) suggests that MAD2 has distinct roles in protein transport and the mitotic feedback control.",
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N2 - THE mad2-1 mutation inactivates the cell-cycle feedback control that prevents budding yeast cells from leaving mitosis until spindle assembly is complete. The gene product of MAD2 shows significant sequence similarity to the α-subunit of prenyltransferases. Here we isolate a new temperature- sensitive mad2 mutant, mad2-2(tx), and find that MAD2p is required for the membrane association of Ypt1p and Sec4p, two prenylated small GTP-binding proteins involved in protein trafficking. Extracts from mad2-2(tx) mutant cells fail to geranylgeranylate a number of substrates at the non-permissive temperature. mad2-2(tx) is synthetically lethal with bet2-1, a mutation in the gene that encodes for the β-subunit of the Ypt1p and Sec4p geranylgeranyl transferase. Therefore MAD2 and BET2 gene products may physically interact to form a geranylgeranyl transferase complex. In addition, the difference between the phenotypes of mad2-1 and mad2-2(tx) suggests that MAD2 has distinct roles in protein transport and the mitotic feedback control.

AB - THE mad2-1 mutation inactivates the cell-cycle feedback control that prevents budding yeast cells from leaving mitosis until spindle assembly is complete. The gene product of MAD2 shows significant sequence similarity to the α-subunit of prenyltransferases. Here we isolate a new temperature- sensitive mad2 mutant, mad2-2(tx), and find that MAD2p is required for the membrane association of Ypt1p and Sec4p, two prenylated small GTP-binding proteins involved in protein trafficking. Extracts from mad2-2(tx) mutant cells fail to geranylgeranylate a number of substrates at the non-permissive temperature. mad2-2(tx) is synthetically lethal with bet2-1, a mutation in the gene that encodes for the β-subunit of the Ypt1p and Sec4p geranylgeranyl transferase. Therefore MAD2 and BET2 gene products may physically interact to form a geranylgeranyl transferase complex. In addition, the difference between the phenotypes of mad2-1 and mad2-2(tx) suggests that MAD2 has distinct roles in protein transport and the mitotic feedback control.

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