The mitochondrial K-ATP channel opener, diazoxide, prevents ischemia-reperfusion injury in the rabbit spinal cord

Glen Roseborough, Daqing Gao, Lei Chen, Michael A. Trush, Shaoyu Zhou, G. Melville Williams, Chiming Wei

Research output: Contribution to journalArticle

Abstract

Paraplegia resulting from ischemia is a catastrophic complication of thoracoabdominal aortic surgery. The current study was designed to Investigate the effects of diazoxide (DZ) on mitochondrial structure, neurological function, DNA damage-repair, and apoptosis in spinal cord ischemia-reperfusion infury. Rabbits were subjected to 30 minutes of spinal cord ischemia and reperfusion (1 hour) with or without diazoxide (n = 6 in each group) by clamping and releasing the infrarenal aorta. The neurological functional score was significantly improved in the DZ-treated ischemia-reperfusion injury group. Electron microscopic studies demonstrated that mitochondrial damage in the spinal cord after injury was significantly reduced by DZ. Mitochondrial superoxide and hydrogen peroxide levels were also markedly decreased in the DZ-treated infury group compared with the untreated group. DZ decreased levels of the oxidative DNA damage product 8-oxoG and increased levels of the DNA repair enzyme OGG-1. Furthermore, DZ inhibited apoptosis via caspase-dependent and -independent pathways. These studies indicate for die first time that the mitochondrial K-ATP channel opener diazoxide improves neurological function after spinal cord ischemia and reperfusion by diminishing levels of reactive oxygen species, decreasing DNA oxidative damage, and inhibiting caspase-dependent and -independent apoptotic pathways while preserving mitochondrial structure.

Original languageEnglish (US)
Pages (from-to)1443-1451
Number of pages9
JournalAmerican Journal of Pathology
Volume168
Issue number5
DOIs
StatePublished - May 2006

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Diazoxide
Reperfusion Injury
Spinal Cord
Rabbits
Spinal Cord Ischemia
DNA Damage
Reperfusion
Caspases
Apoptosis
DNA Repair Enzymes
mitochondrial K(ATP) channel
Paraplegia
Spinal Cord Injuries
Constriction
Superoxides
DNA Repair
Hydrogen Peroxide
Aorta
Reactive Oxygen Species
Ischemia

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

The mitochondrial K-ATP channel opener, diazoxide, prevents ischemia-reperfusion injury in the rabbit spinal cord. / Roseborough, Glen; Gao, Daqing; Chen, Lei; Trush, Michael A.; Zhou, Shaoyu; Williams, G. Melville; Wei, Chiming.

In: American Journal of Pathology, Vol. 168, No. 5, 05.2006, p. 1443-1451.

Research output: Contribution to journalArticle

Roseborough, Glen ; Gao, Daqing ; Chen, Lei ; Trush, Michael A. ; Zhou, Shaoyu ; Williams, G. Melville ; Wei, Chiming. / The mitochondrial K-ATP channel opener, diazoxide, prevents ischemia-reperfusion injury in the rabbit spinal cord. In: American Journal of Pathology. 2006 ; Vol. 168, No. 5. pp. 1443-1451.
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