The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1

Brian E. Reese, Kurtis E. Bachman, Stephen B. Baylin, Michael R. Rountree

Research output: Contribution to journalArticlepeer-review

Abstract

DNA promoter hypermethylation has been shown to be a functional mechanism of transcriptional repression. This epigenetic gene silencing is thought to involve the recruitment of chromatin-remodeling factors, such as histone deacetylases, to methylated DNA via a family of proteins called methyl-CpG binding proteins (MBD1 to -4). MBD1, a member of this family, exhibits transcription-repressive activity, but to this point no interacting protein partners have been identified. In this study, we demonstrate that MBD1 partners with the p150 subunit of chromatin assembly factor 1 (CAF-1), forming a multiprotein complex that also contains HP1α. The MBD1-CAF-1 p150 interaction requires the methyl-CpG binding domain of MBD1, and the association occurs in the C terminus of CAF-1 p150. The two proteins colocalize to regions of dense heterochromatin in mouse cells, and overexpression of the C terminus of CAF-1 p150 prevents the targeting of MBD1 in these structure. This interaction suggests a role for MBD1 and CAF-1 p150 in methylation-mediated transcriptional repression and the inheritance of epigenetically determined chromatin states.

Original languageEnglish (US)
Pages (from-to)3226-3236
Number of pages11
JournalMolecular and cellular biology
Volume23
Issue number9
DOIs
StatePublished - May 2003

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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