The methodology for determining the efficacy of antibody-mediated immunity

Research output: Contribution to journalArticle

Abstract

The basic method for evaluating the efficacy of antibody-mediated immunity (AMI) dates from the 1890s and involves the administration of specific Ab to an immunologically naïve host followed by microbial or toxin challenge. Other methods used to evaluate AMI involve correlating the presence of specific Ab with resistance to microbial disease and associating susceptibility to certain microbes with host immunoglobulin deficits. Unfortunately, each method has theoretical and practical problems that limit their usefulness when negative results are obtained. The application of hybridoma technology to investigate the efficacy of AMI has shown that it is possible to generate protective monoclonal antibodies even to microbes for which the standard methodologies indicate no role for AMI. Furthermore, studies with monoclonal antibodies suggest various explanations for the inability of standard methods to demonstrate the potential efficacy of AMI for certain pathogens.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalJournal of Immunological Methods
Volume291
Issue number1-2
DOIs
StatePublished - Aug 2004
Externally publishedYes

Fingerprint

Immunity
Antibodies
Monoclonal Antibodies
Disease Susceptibility
Hybridomas
Immunoglobulins
Technology

Keywords

  • Animal models
  • Antibody
  • Method
  • Passive
  • Protection

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

The methodology for determining the efficacy of antibody-mediated immunity. / Casadevall, Arturo.

In: Journal of Immunological Methods, Vol. 291, No. 1-2, 08.2004, p. 1-10.

Research output: Contribution to journalArticle

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