Abstract
The basic method for evaluating the efficacy of antibody-mediated immunity (AMI) dates from the 1890s and involves the administration of specific Ab to an immunologically naïve host followed by microbial or toxin challenge. Other methods used to evaluate AMI involve correlating the presence of specific Ab with resistance to microbial disease and associating susceptibility to certain microbes with host immunoglobulin deficits. Unfortunately, each method has theoretical and practical problems that limit their usefulness when negative results are obtained. The application of hybridoma technology to investigate the efficacy of AMI has shown that it is possible to generate protective monoclonal antibodies even to microbes for which the standard methodologies indicate no role for AMI. Furthermore, studies with monoclonal antibodies suggest various explanations for the inability of standard methods to demonstrate the potential efficacy of AMI for certain pathogens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1-10 |
| Number of pages | 10 |
| Journal | Journal of Immunological Methods |
| Volume | 291 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Aug 2004 |
| Externally published | Yes |
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Keywords
- Animal models
- Antibody
- Method
- Passive
- Protection
ASJC Scopus subject areas
- Biotechnology
- Immunology
Cite this
The methodology for determining the efficacy of antibody-mediated immunity. / Casadevall, Arturo.
In: Journal of Immunological Methods, Vol. 291, No. 1-2, 08.2004, p. 1-10.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The methodology for determining the efficacy of antibody-mediated immunity
AU - Casadevall, Arturo
PY - 2004/8
Y1 - 2004/8
N2 - The basic method for evaluating the efficacy of antibody-mediated immunity (AMI) dates from the 1890s and involves the administration of specific Ab to an immunologically naïve host followed by microbial or toxin challenge. Other methods used to evaluate AMI involve correlating the presence of specific Ab with resistance to microbial disease and associating susceptibility to certain microbes with host immunoglobulin deficits. Unfortunately, each method has theoretical and practical problems that limit their usefulness when negative results are obtained. The application of hybridoma technology to investigate the efficacy of AMI has shown that it is possible to generate protective monoclonal antibodies even to microbes for which the standard methodologies indicate no role for AMI. Furthermore, studies with monoclonal antibodies suggest various explanations for the inability of standard methods to demonstrate the potential efficacy of AMI for certain pathogens.
AB - The basic method for evaluating the efficacy of antibody-mediated immunity (AMI) dates from the 1890s and involves the administration of specific Ab to an immunologically naïve host followed by microbial or toxin challenge. Other methods used to evaluate AMI involve correlating the presence of specific Ab with resistance to microbial disease and associating susceptibility to certain microbes with host immunoglobulin deficits. Unfortunately, each method has theoretical and practical problems that limit their usefulness when negative results are obtained. The application of hybridoma technology to investigate the efficacy of AMI has shown that it is possible to generate protective monoclonal antibodies even to microbes for which the standard methodologies indicate no role for AMI. Furthermore, studies with monoclonal antibodies suggest various explanations for the inability of standard methods to demonstrate the potential efficacy of AMI for certain pathogens.
KW - Animal models
KW - Antibody
KW - Method
KW - Passive
KW - Protection
UR - http://www.scopus.com/inward/record.url?scp=4444290709&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4444290709&partnerID=8YFLogxK
U2 - 10.1016/j.jim.2004.04.027
DO - 10.1016/j.jim.2004.04.027
M3 - Article
VL - 291
SP - 1
EP - 10
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1-2
ER -