The mechanochemistry of endocytosis

Jian Liu, Yidi Sun, David G. Drubin, George F. Oster

Research output: Contribution to journalArticle

Abstract

Endocytic vesicle formation is a complex process that couples sequential protein recruitment and lipid modifications with dramatic shape transformations of the plasma membrane. Although individual molecular players have been studied intensively, how they all fit into a coherent picture of endocytosis remains unclear. That is, how the proper temporal and spatial coordination of endocytic events is achieved and what drives vesicle scission are not known. Drawing upon detailed knowledge from experiments in yeast, we develop the first integrated mechanochemical model that quantitatively recapitulates the temporal and spatial progression of endocytic events leading to vesicle scission. The central idea is that membrane curvature is coupled to the accompanying biochemical reactions. This coupling ensures that the process is robust and culminates in an interfacial force that pinches off the vesicle. Calculated phase diagrams reproduce endocytic mutant phenotypes observed in experiments and predict unique testable endocytic phenotypes in yeast and mammalian cells. The combination of experiments and theory in this work suggest a unified mechanism for endocytic vesicle formation across eukaryotes.

Original languageEnglish (US)
Article numbere1000204
JournalPLoS Biology
Volume7
Issue number9
DOIs
StatePublished - Oct 14 2009
Externally publishedYes

Fingerprint

Transport Vesicles
endocytosis
Endocytosis
Yeasts
yeasts
Phenotype
phenotype
Yeast
Surface Tension
surface tension
chemical reactions
Eukaryota
eukaryotic cells
plasma membrane
Experiments
Cell Membrane
Cell membranes
Lipids
mutants
Phase diagrams

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Liu, J., Sun, Y., Drubin, D. G., & Oster, G. F. (2009). The mechanochemistry of endocytosis. PLoS Biology, 7(9), [e1000204]. https://doi.org/10.1371/journal.pbio.1000204

The mechanochemistry of endocytosis. / Liu, Jian; Sun, Yidi; Drubin, David G.; Oster, George F.

In: PLoS Biology, Vol. 7, No. 9, e1000204, 14.10.2009.

Research output: Contribution to journalArticle

Liu, J, Sun, Y, Drubin, DG & Oster, GF 2009, 'The mechanochemistry of endocytosis', PLoS Biology, vol. 7, no. 9, e1000204. https://doi.org/10.1371/journal.pbio.1000204
Liu, Jian ; Sun, Yidi ; Drubin, David G. ; Oster, George F. / The mechanochemistry of endocytosis. In: PLoS Biology. 2009 ; Vol. 7, No. 9.
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