The mechanism of microtubule dependent movement of pigment granules in teleost chromatophores

D. B. Murphy

Research output: Contribution to journalArticle

Abstract

The relationship of cytoplasmic microtubules to intracellular transport was investigated in teleost chromatophores. Antimitotic agents, including colchicine, vinblastine, hydrostatic pressure, and low temperature remove microtubules in these cells, and simultaneously disrupt the alignment and arrest the movement of pigment granules. During recovery, the return of alignment and movement corresponds in both time and space with the repolymerization of microtubules. These results demonstrate that microtubules are essential for the intracellular transport of pigment. Investigations of the mechanism of movement show that microtubules do not change in number or location during the redistribution of pigment in Fundulus melanophores. Microtubules in melanophores also behave as semistable organelles as determined by investigation with colchicine and hydrostatic pressure. These observations and others rule out a push pull mechanism based on the polymerization and depolymerization of microtubules or one that distinguishes 2 operationally different sets of microtubules. It is proposed instead that particles move by sliding along a fixed array of microtubules.

Original languageEnglish (US)
Pages (from-to)692-701
Number of pages10
JournalAnnals of the New York Academy of Sciences
VolumeVol. 253
StatePublished - 1975
Externally publishedYes

Fingerprint

Chromatophores
Pigments
Microtubules
Colchicine
Hydrostatic pressure
Antimitotic Agents
Depolymerization
Vinblastine
Melanophores
Hydrostatic Pressure
Polymerization
Recovery
Fundulidae
Organelles
Temperature

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The mechanism of microtubule dependent movement of pigment granules in teleost chromatophores. / Murphy, D. B.

In: Annals of the New York Academy of Sciences, Vol. Vol. 253, 1975, p. 692-701.

Research output: Contribution to journalArticle

@article{db1e1b82caaf4214b44d7ebe508d9d5f,
title = "The mechanism of microtubule dependent movement of pigment granules in teleost chromatophores",
abstract = "The relationship of cytoplasmic microtubules to intracellular transport was investigated in teleost chromatophores. Antimitotic agents, including colchicine, vinblastine, hydrostatic pressure, and low temperature remove microtubules in these cells, and simultaneously disrupt the alignment and arrest the movement of pigment granules. During recovery, the return of alignment and movement corresponds in both time and space with the repolymerization of microtubules. These results demonstrate that microtubules are essential for the intracellular transport of pigment. Investigations of the mechanism of movement show that microtubules do not change in number or location during the redistribution of pigment in Fundulus melanophores. Microtubules in melanophores also behave as semistable organelles as determined by investigation with colchicine and hydrostatic pressure. These observations and others rule out a push pull mechanism based on the polymerization and depolymerization of microtubules or one that distinguishes 2 operationally different sets of microtubules. It is proposed instead that particles move by sliding along a fixed array of microtubules.",
author = "Murphy, {D. B.}",
year = "1975",
language = "English (US)",
volume = "Vol. 253",
pages = "692--701",
journal = "Annals of the New York Academy of Sciences",
issn = "0077-8923",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - The mechanism of microtubule dependent movement of pigment granules in teleost chromatophores

AU - Murphy, D. B.

PY - 1975

Y1 - 1975

N2 - The relationship of cytoplasmic microtubules to intracellular transport was investigated in teleost chromatophores. Antimitotic agents, including colchicine, vinblastine, hydrostatic pressure, and low temperature remove microtubules in these cells, and simultaneously disrupt the alignment and arrest the movement of pigment granules. During recovery, the return of alignment and movement corresponds in both time and space with the repolymerization of microtubules. These results demonstrate that microtubules are essential for the intracellular transport of pigment. Investigations of the mechanism of movement show that microtubules do not change in number or location during the redistribution of pigment in Fundulus melanophores. Microtubules in melanophores also behave as semistable organelles as determined by investigation with colchicine and hydrostatic pressure. These observations and others rule out a push pull mechanism based on the polymerization and depolymerization of microtubules or one that distinguishes 2 operationally different sets of microtubules. It is proposed instead that particles move by sliding along a fixed array of microtubules.

AB - The relationship of cytoplasmic microtubules to intracellular transport was investigated in teleost chromatophores. Antimitotic agents, including colchicine, vinblastine, hydrostatic pressure, and low temperature remove microtubules in these cells, and simultaneously disrupt the alignment and arrest the movement of pigment granules. During recovery, the return of alignment and movement corresponds in both time and space with the repolymerization of microtubules. These results demonstrate that microtubules are essential for the intracellular transport of pigment. Investigations of the mechanism of movement show that microtubules do not change in number or location during the redistribution of pigment in Fundulus melanophores. Microtubules in melanophores also behave as semistable organelles as determined by investigation with colchicine and hydrostatic pressure. These observations and others rule out a push pull mechanism based on the polymerization and depolymerization of microtubules or one that distinguishes 2 operationally different sets of microtubules. It is proposed instead that particles move by sliding along a fixed array of microtubules.

UR - http://www.scopus.com/inward/record.url?scp=0016586584&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016586584&partnerID=8YFLogxK

M3 - Article

C2 - 1056758

AN - SCOPUS:0016586584

VL - Vol. 253

SP - 692

EP - 701

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

SN - 0077-8923

ER -