The mechanism of glucose 6-phosphate transport by Escherichia coli

L. A. Sonna, S. V. Ambudkar, P. C. Maloney

Research output: Contribution to journalArticlepeer-review

Abstract

To evaluate anion exchange as the mechanistic basis of sugar phosphate transport, natural and artificial membranes were used in studies of glucose 6-phosphate (Glc-6-P) and inorganic phosphate (P(i)) accumulation by the uhpT-encoded protein (UhpT) of Escherichia coli. Experiments with intact cells demonstrated that UhpT catalyzed the neutral exchange of internal and external P(i), and work with everted as well as right-side-out membrane vesicles showed further that UhpT mediated the heterologous exchange of P(i) and Glc-6-P. When loaded with P(i), but not when loaded with morpholinopropanesulfonate (MOPS), everted vesicles took up Glc-6-P to levels 100-fold above medium concentration in a reaction unaffected by the ionophores valinomycin, valinomycin plus nigericin, and carbonyl cyanide p-trifluoromethoxyphenylhydrazone. Similarly, right-side-out vesicles were capable of Glc-6-P transport, but only if a suitable internal countersubstrate was available. Thus, in MOPS-loaded vesicles, oxidative metabolism established a proton-motive force that supported proline or P(i) accumulation, but transport of Glc-6-P was found only if vesicles could accumulate P(i) during a preincubation. After reconstitution of UhpT into proteoliposomes it was possible to show as well that the level of accumulation of Glc-6-P (17 to 560 nmol/mg of protein) was related directly to the internal concentration of P(i). These results are most easily understood if the transport of glucose 6-phosphate in E. coli occurs by anion exchange rather than by nH+/anion symport.

Original languageEnglish (US)
Pages (from-to)6625-6630
Number of pages6
JournalJournal of Biological Chemistry
Volume263
Issue number14
StatePublished - 1988

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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