The measurement of androgen receptors in human prostatic tissue utilizing sucrose density centrifugation and a protamine precipitation assay

M. Menon, C. E. Tananis, M. G. McLoughlin, M. E. Lippman, Patrick Walsh

Research output: Contribution to journalArticle

Abstract

Methods have been established for the measurement of androgen receptors in the cytosol and crude nuclear extract of human prostatic tissue using sucrose density gradient centrifugation and a protamine precipitation assay. These studies have demonstrated the presence of a high affinity, heat labile protein that binds preferentially to dihydrotestosterone and that is present only in androgen target tissues. In studies of prostatic tissue from 15 patients sucrose density gradient centrifugation appeared more sensitive than the protamine precipitation assay in the identification of androgen receptors. These findings appear promising and suggest that in the near future a reliable assay may be available for the measurement of androgen receptors in prostatic carcinoma.

Original languageEnglish (US)
Pages (from-to)309-312
Number of pages4
JournalJournal of Urology
Volume117
Issue number3
StatePublished - 1977
Externally publishedYes

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Protamines
Androgen Receptors
Centrifugation
Sucrose
Density Gradient Centrifugation
Dihydrotestosterone
Complex Mixtures
Cytosol
Androgens
Hot Temperature
Carcinoma
human AR protein
Proteins

ASJC Scopus subject areas

  • Urology

Cite this

The measurement of androgen receptors in human prostatic tissue utilizing sucrose density centrifugation and a protamine precipitation assay. / Menon, M.; Tananis, C. E.; McLoughlin, M. G.; Lippman, M. E.; Walsh, Patrick.

In: Journal of Urology, Vol. 117, No. 3, 1977, p. 309-312.

Research output: Contribution to journalArticle

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