TY - JOUR
T1 - The maturation of GABAergic transmission in visual cortex requires endocannabinoid-mediated LTD of inhibitory inputs during a critical period
AU - Jiang, Bin
AU - Huang, Shiyong
AU - de Pasquale, Roberto
AU - Millman, Daniel
AU - Song, Lihua
AU - Lee, Hey Kyoung
AU - Tsumoto Tadaharu, T.
AU - Kirkwood Alfredo, A.
PY - 2010/4
Y1 - 2010/4
N2 - Endocannabinoids are widely regarded as negative modulators of presynaptic release. Here, we present evidence that in visual cortex endocannabinoids are crucial for the maturation of GABAergic release. We found that between eye opening and puberty, release changes from an immature state with high release probability, short-term depression (STD), and high release variability during irregular patterned activity, to a mature state with reduced release probability, STD, and variability. This transition requires visual experience and stimulation of CB1 cannabinoid receptors as it is mimicked by administration of CB1 agonists, blocked by antagonists, and is absent in CB1R KO mice. In immature slices, activation of CB1 receptors induces long-term depression of inhibitory responses (iLTD) and a reduction in STD and response variability. Based on these findings, we propose that visually induced endocannabinoid-dependent iLTD mediates the developmental decrease in release probability, STD, and response variability, which are characteristic of maturation of cortical GABAergic inhibition.
AB - Endocannabinoids are widely regarded as negative modulators of presynaptic release. Here, we present evidence that in visual cortex endocannabinoids are crucial for the maturation of GABAergic release. We found that between eye opening and puberty, release changes from an immature state with high release probability, short-term depression (STD), and high release variability during irregular patterned activity, to a mature state with reduced release probability, STD, and variability. This transition requires visual experience and stimulation of CB1 cannabinoid receptors as it is mimicked by administration of CB1 agonists, blocked by antagonists, and is absent in CB1R KO mice. In immature slices, activation of CB1 receptors induces long-term depression of inhibitory responses (iLTD) and a reduction in STD and response variability. Based on these findings, we propose that visually induced endocannabinoid-dependent iLTD mediates the developmental decrease in release probability, STD, and response variability, which are characteristic of maturation of cortical GABAergic inhibition.
KW - Cellbio
KW - Devbio
KW - Sysneuro
UR - http://www.scopus.com/inward/record.url?scp=77952394021&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77952394021&partnerID=8YFLogxK
U2 - 10.1016/j.neuron.2010.03.021
DO - 10.1016/j.neuron.2010.03.021
M3 - Article
C2 - 20435001
AN - SCOPUS:77952394021
SN - 0896-6273
VL - 66
SP - 248
EP - 259
JO - Neuron
JF - Neuron
IS - 2
ER -