The mammalian target of rapamycin (mTOR) pathway regulates mitochondrial oxygen consumption and oxidative capacity

Stefan M. Schieke, Darci Phillips, J. Philip McCoy, Angel M. Aponte, Rong Fong Shen, Robert S. Balaban, Toren Finkel

Research output: Contribution to journalArticle

Abstract

Metabolic rate and the subsequent production of reactive oxygen species are thought to contribute to the rate of aging in a wide range of species. The target of rapamycin (TOR) is a well conserved serine/threonine kinase that regulates cell growth in response to nutrient status. Here we demonstrate that in mammalian cells the mammalian TOR (mTOR) pathway plays a significant role in determining both resting oxygen consumption and oxidative capacity. In particular, we demonstrate that the level of complex formation between mTOR and one of its known protein partners, raptor, correlated with overall mitochondrial activity. Disruption of this complex following treatment with the mTOR pharmacological inhibitor rapamycin lowered mitochondrial membrane potential, oxygen consumption, and ATP synthetic capacity. Subcellular fractionation revealed that mTOR as well as mTOR-raptor complexes can be purified in the mitochondrial fraction. Using two-dimensional difference gel electrophoresis, we further demonstrated that inhibiting mTOR with rapamycin resulted in a dramatic alteration in the mitochondrial phosphoproteome. RNA interference-mediated knockdown of TSC2, p70 S6 kinase (S6K1), raptor, or rictor demonstrates that mTOR regulates mitochondrial activity independently of its previously identified cellular targets. Finally we demonstrate that mTOR activity may play an important role in determining the relative balance between mitochondrial and non-mitochondrial sources of ATP generation. These results may provide insight into recent observations linking the TOR pathway to life span regulation of lower organisms.

Original languageEnglish (US)
Pages (from-to)27643-27652
Number of pages10
JournalJournal of Biological Chemistry
Volume281
Issue number37
DOIs
StatePublished - Sep 15 2006
Externally publishedYes

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Sirolimus
Oxygen Consumption
Oxygen
Raptors
Adenosine Triphosphate
Two-Dimensional Difference Gel Electrophoresis
70-kDa Ribosomal Protein S6 Kinases
Mitochondrial Membrane Potential
Protein-Serine-Threonine Kinases
Cell growth
Fractionation
RNA Interference
Electrophoresis
Nutrients
Reactive Oxygen Species
Aging of materials
Gels
Cells
Pharmacology
RNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Schieke, S. M., Phillips, D., McCoy, J. P., Aponte, A. M., Shen, R. F., Balaban, R. S., & Finkel, T. (2006). The mammalian target of rapamycin (mTOR) pathway regulates mitochondrial oxygen consumption and oxidative capacity. Journal of Biological Chemistry, 281(37), 27643-27652. https://doi.org/10.1074/jbc.M603536200

The mammalian target of rapamycin (mTOR) pathway regulates mitochondrial oxygen consumption and oxidative capacity. / Schieke, Stefan M.; Phillips, Darci; McCoy, J. Philip; Aponte, Angel M.; Shen, Rong Fong; Balaban, Robert S.; Finkel, Toren.

In: Journal of Biological Chemistry, Vol. 281, No. 37, 15.09.2006, p. 27643-27652.

Research output: Contribution to journalArticle

Schieke, SM, Phillips, D, McCoy, JP, Aponte, AM, Shen, RF, Balaban, RS & Finkel, T 2006, 'The mammalian target of rapamycin (mTOR) pathway regulates mitochondrial oxygen consumption and oxidative capacity', Journal of Biological Chemistry, vol. 281, no. 37, pp. 27643-27652. https://doi.org/10.1074/jbc.M603536200
Schieke, Stefan M. ; Phillips, Darci ; McCoy, J. Philip ; Aponte, Angel M. ; Shen, Rong Fong ; Balaban, Robert S. ; Finkel, Toren. / The mammalian target of rapamycin (mTOR) pathway regulates mitochondrial oxygen consumption and oxidative capacity. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 37. pp. 27643-27652.
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