The Magnaporthe grisea snodprot1 homolog, MSP1, is required for virulence

Jun Seop Jeong, Thomas K. Mitchell, Ralph A. Dean

Research output: Contribution to journalArticle

Abstract

Secreted proteins play central roles in plant-microbe interactions acting as signals, toxins, and effectors. One important group of small secreted proteins is the snodprot1 family, members of which have demonstrated phytotoxic properties. A split-marker transformation system was applied for gene deletion of the snodprot1 homolog, MSP1, in the rice blast fungus Magnaporthe grisea. msp1 mutants were phenotypically indistinguishable from wild type and elaborated apparently normal appressoria. However, the deletion mutants were greatly reduced in virulence primarily due to impaired growth in planta. Western blot analysis showed that the protein was secreted and not associated with the fungal cell wall. When purified MSP1 protein was applied to wounded leaf tissue, no apparent phytotoxic effects were noted. This is the first report to the authors' knowledge that directly implicates a snodprot1 protein as a virulence factor.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalFEMS Microbiology Letters
Volume273
Issue number2
DOIs
StatePublished - Aug 2007
Externally publishedYes

Fingerprint

Merozoite Surface Protein 1
Magnaporthe
Virulence
Proteins
Gene Deletion
Virulence Factors
Cell Wall
Fungi
Western Blotting
Growth

Keywords

  • Hydrophobin
  • Infection
  • Pathogenicity
  • Rice
  • Rice blast

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

The Magnaporthe grisea snodprot1 homolog, MSP1, is required for virulence. / Jeong, Jun Seop; Mitchell, Thomas K.; Dean, Ralph A.

In: FEMS Microbiology Letters, Vol. 273, No. 2, 08.2007, p. 157-165.

Research output: Contribution to journalArticle

Jeong, Jun Seop ; Mitchell, Thomas K. ; Dean, Ralph A. / The Magnaporthe grisea snodprot1 homolog, MSP1, is required for virulence. In: FEMS Microbiology Letters. 2007 ; Vol. 273, No. 2. pp. 157-165.
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