The macrophage disappearance reaction (MDR) as an in vivo test of delayed hypersensitivity in mice

Vesna Tomazic, Pierluigi E. Bigazzi, Noel R. Rose

Research output: Contribution to journalArticle

Abstract

The macrophage disappearance reaction (MDR), as an in vivo analogue of in vitro tests for delayed hypersensitivity, was utilized in mice immunized with M. tuberculosis or mouse thyroid extract (MTE). The optimal schedule for the induction of macrophages in peritoneal exudates and the optimal concentration of antigen for the MDR were determined. Macrophage disappearance occurred 4 hr after immunized mice received an intraperitoneal injection of 200 μg of soluble antigen. The MDR was found to be antigen specific. Intraperitoneal injection of an unrelated antigen or tissue culture medium alone did not cause macrophage disappearance; however, the re-injection of the antigen used for immunization caused a 70-80% reduction of macrophages. Macrophage disappearance was greater in mice immunized with M. tuberculosis than in mice immunized with MTE. Comparison of the MDR with the footpad test in these 2 groups showed that mice immunized with M. tuberculosis developed a higher degree of delayed hypersensitivity than the group immunized with MTE. These results demonstrate that the MDR represents a specific and quantitative method for detecting the delayed type of cellular immune response in mice.

Original languageEnglish (US)
Pages (from-to)49-62
Number of pages14
JournalImmunological Investigations
Volume6
Issue number1
DOIs
StatePublished - 1977
Externally publishedYes

Fingerprint

Delayed Hypersensitivity
Macrophages
Antigens
Thyroid Gland
Tuberculosis
Intraperitoneal Injections
Peritoneal Macrophages
Exudates and Transudates
Cellular Immunity
Culture Media
Immunization
Appointments and Schedules
Injections

ASJC Scopus subject areas

  • Immunology

Cite this

The macrophage disappearance reaction (MDR) as an in vivo test of delayed hypersensitivity in mice. / Tomazic, Vesna; Bigazzi, Pierluigi E.; Rose, Noel R.

In: Immunological Investigations, Vol. 6, No. 1, 1977, p. 49-62.

Research output: Contribution to journalArticle

Tomazic, Vesna ; Bigazzi, Pierluigi E. ; Rose, Noel R. / The macrophage disappearance reaction (MDR) as an in vivo test of delayed hypersensitivity in mice. In: Immunological Investigations. 1977 ; Vol. 6, No. 1. pp. 49-62.
@article{c907f07a52e0484195903cbf80e334cf,
title = "The macrophage disappearance reaction (MDR) as an in vivo test of delayed hypersensitivity in mice",
abstract = "The macrophage disappearance reaction (MDR), as an in vivo analogue of in vitro tests for delayed hypersensitivity, was utilized in mice immunized with M. tuberculosis or mouse thyroid extract (MTE). The optimal schedule for the induction of macrophages in peritoneal exudates and the optimal concentration of antigen for the MDR were determined. Macrophage disappearance occurred 4 hr after immunized mice received an intraperitoneal injection of 200 μg of soluble antigen. The MDR was found to be antigen specific. Intraperitoneal injection of an unrelated antigen or tissue culture medium alone did not cause macrophage disappearance; however, the re-injection of the antigen used for immunization caused a 70-80{\%} reduction of macrophages. Macrophage disappearance was greater in mice immunized with M. tuberculosis than in mice immunized with MTE. Comparison of the MDR with the footpad test in these 2 groups showed that mice immunized with M. tuberculosis developed a higher degree of delayed hypersensitivity than the group immunized with MTE. These results demonstrate that the MDR represents a specific and quantitative method for detecting the delayed type of cellular immune response in mice.",
author = "Vesna Tomazic and Bigazzi, {Pierluigi E.} and Rose, {Noel R.}",
year = "1977",
doi = "10.3109/08820137709055803",
language = "English (US)",
volume = "6",
pages = "49--62",
journal = "Immunological Investigations",
issn = "0882-0139",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - The macrophage disappearance reaction (MDR) as an in vivo test of delayed hypersensitivity in mice

AU - Tomazic, Vesna

AU - Bigazzi, Pierluigi E.

AU - Rose, Noel R.

PY - 1977

Y1 - 1977

N2 - The macrophage disappearance reaction (MDR), as an in vivo analogue of in vitro tests for delayed hypersensitivity, was utilized in mice immunized with M. tuberculosis or mouse thyroid extract (MTE). The optimal schedule for the induction of macrophages in peritoneal exudates and the optimal concentration of antigen for the MDR were determined. Macrophage disappearance occurred 4 hr after immunized mice received an intraperitoneal injection of 200 μg of soluble antigen. The MDR was found to be antigen specific. Intraperitoneal injection of an unrelated antigen or tissue culture medium alone did not cause macrophage disappearance; however, the re-injection of the antigen used for immunization caused a 70-80% reduction of macrophages. Macrophage disappearance was greater in mice immunized with M. tuberculosis than in mice immunized with MTE. Comparison of the MDR with the footpad test in these 2 groups showed that mice immunized with M. tuberculosis developed a higher degree of delayed hypersensitivity than the group immunized with MTE. These results demonstrate that the MDR represents a specific and quantitative method for detecting the delayed type of cellular immune response in mice.

AB - The macrophage disappearance reaction (MDR), as an in vivo analogue of in vitro tests for delayed hypersensitivity, was utilized in mice immunized with M. tuberculosis or mouse thyroid extract (MTE). The optimal schedule for the induction of macrophages in peritoneal exudates and the optimal concentration of antigen for the MDR were determined. Macrophage disappearance occurred 4 hr after immunized mice received an intraperitoneal injection of 200 μg of soluble antigen. The MDR was found to be antigen specific. Intraperitoneal injection of an unrelated antigen or tissue culture medium alone did not cause macrophage disappearance; however, the re-injection of the antigen used for immunization caused a 70-80% reduction of macrophages. Macrophage disappearance was greater in mice immunized with M. tuberculosis than in mice immunized with MTE. Comparison of the MDR with the footpad test in these 2 groups showed that mice immunized with M. tuberculosis developed a higher degree of delayed hypersensitivity than the group immunized with MTE. These results demonstrate that the MDR represents a specific and quantitative method for detecting the delayed type of cellular immune response in mice.

UR - http://www.scopus.com/inward/record.url?scp=84907105816&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907105816&partnerID=8YFLogxK

U2 - 10.3109/08820137709055803

DO - 10.3109/08820137709055803

M3 - Article

AN - SCOPUS:84907105816

VL - 6

SP - 49

EP - 62

JO - Immunological Investigations

JF - Immunological Investigations

SN - 0882-0139

IS - 1

ER -