The LINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransduction

Allison B. Chambliss, Shyam B. Khatau, Nicholas Erdenberger, D. Kyle Robinson, Didier Hodzic, Gregory D. Longmore, Denis Wirtz

Research output: Contribution to journalArticle

Abstract

Cells continuously sense and respond to external mechanical forces through their cytoskeleton. Here we show that only a small subset of actin fibers, those forming the perinuclear actin cap that wraps around the nucleus, form in response to low physiological mechanical stresses in adherent fibroblasts. While conventional basal stress fibers form only past a threshold shear stress of 0.5 dyn/cm2, actin-cap fibers are formed at shear stresses 50 times lower and orders-of-magnitude faster than biochemical stimulation. This fast differential response is uniquely mediated by focal adhesion protein zyxin at low shear stress and actomyosin fibers of the actin cap. We identify additional roles for lamin A/C of the nuclear lamina and linkers of nucleus to cytoskeleton (LINC) molecules nesprin2giant and nesprin3, which anchor actin cap fibers to the nucleus. These results suggest an interconnected physical pathway for mechanotransduction, from the extracellular milieu to the nucleus.

Original languageEnglish (US)
Article number1087
JournalScientific Reports
Volume3
DOIs
StatePublished - Jan 18 2013

Fingerprint

Actin Cytoskeleton
Actins
Stress Fibers
Cytoskeleton
Zyxin
Nuclear Lamina
Lamin Type A
Actomyosin
Mechanical Stress
Focal Adhesions
Fibroblasts

ASJC Scopus subject areas

  • General

Cite this

Chambliss, A. B., Khatau, S. B., Erdenberger, N., Robinson, D. K., Hodzic, D., Longmore, G. D., & Wirtz, D. (2013). The LINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransduction. Scientific Reports, 3, [1087]. https://doi.org/10.1038/srep01087

The LINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransduction. / Chambliss, Allison B.; Khatau, Shyam B.; Erdenberger, Nicholas; Robinson, D. Kyle; Hodzic, Didier; Longmore, Gregory D.; Wirtz, Denis.

In: Scientific Reports, Vol. 3, 1087, 18.01.2013.

Research output: Contribution to journalArticle

Chambliss, Allison B. ; Khatau, Shyam B. ; Erdenberger, Nicholas ; Robinson, D. Kyle ; Hodzic, Didier ; Longmore, Gregory D. ; Wirtz, Denis. / The LINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransduction. In: Scientific Reports. 2013 ; Vol. 3.
@article{e3ffbe7a05c34b979b03fd36093bb86c,
title = "The LINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransduction",
abstract = "Cells continuously sense and respond to external mechanical forces through their cytoskeleton. Here we show that only a small subset of actin fibers, those forming the perinuclear actin cap that wraps around the nucleus, form in response to low physiological mechanical stresses in adherent fibroblasts. While conventional basal stress fibers form only past a threshold shear stress of 0.5 dyn/cm2, actin-cap fibers are formed at shear stresses 50 times lower and orders-of-magnitude faster than biochemical stimulation. This fast differential response is uniquely mediated by focal adhesion protein zyxin at low shear stress and actomyosin fibers of the actin cap. We identify additional roles for lamin A/C of the nuclear lamina and linkers of nucleus to cytoskeleton (LINC) molecules nesprin2giant and nesprin3, which anchor actin cap fibers to the nucleus. These results suggest an interconnected physical pathway for mechanotransduction, from the extracellular milieu to the nucleus.",
author = "Chambliss, {Allison B.} and Khatau, {Shyam B.} and Nicholas Erdenberger and Robinson, {D. Kyle} and Didier Hodzic and Longmore, {Gregory D.} and Denis Wirtz",
year = "2013",
month = "1",
day = "18",
doi = "10.1038/srep01087",
language = "English (US)",
volume = "3",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - The LINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransduction

AU - Chambliss, Allison B.

AU - Khatau, Shyam B.

AU - Erdenberger, Nicholas

AU - Robinson, D. Kyle

AU - Hodzic, Didier

AU - Longmore, Gregory D.

AU - Wirtz, Denis

PY - 2013/1/18

Y1 - 2013/1/18

N2 - Cells continuously sense and respond to external mechanical forces through their cytoskeleton. Here we show that only a small subset of actin fibers, those forming the perinuclear actin cap that wraps around the nucleus, form in response to low physiological mechanical stresses in adherent fibroblasts. While conventional basal stress fibers form only past a threshold shear stress of 0.5 dyn/cm2, actin-cap fibers are formed at shear stresses 50 times lower and orders-of-magnitude faster than biochemical stimulation. This fast differential response is uniquely mediated by focal adhesion protein zyxin at low shear stress and actomyosin fibers of the actin cap. We identify additional roles for lamin A/C of the nuclear lamina and linkers of nucleus to cytoskeleton (LINC) molecules nesprin2giant and nesprin3, which anchor actin cap fibers to the nucleus. These results suggest an interconnected physical pathway for mechanotransduction, from the extracellular milieu to the nucleus.

AB - Cells continuously sense and respond to external mechanical forces through their cytoskeleton. Here we show that only a small subset of actin fibers, those forming the perinuclear actin cap that wraps around the nucleus, form in response to low physiological mechanical stresses in adherent fibroblasts. While conventional basal stress fibers form only past a threshold shear stress of 0.5 dyn/cm2, actin-cap fibers are formed at shear stresses 50 times lower and orders-of-magnitude faster than biochemical stimulation. This fast differential response is uniquely mediated by focal adhesion protein zyxin at low shear stress and actomyosin fibers of the actin cap. We identify additional roles for lamin A/C of the nuclear lamina and linkers of nucleus to cytoskeleton (LINC) molecules nesprin2giant and nesprin3, which anchor actin cap fibers to the nucleus. These results suggest an interconnected physical pathway for mechanotransduction, from the extracellular milieu to the nucleus.

UR - http://www.scopus.com/inward/record.url?scp=84878038512&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878038512&partnerID=8YFLogxK

U2 - 10.1038/srep01087

DO - 10.1038/srep01087

M3 - Article

C2 - 23336069

AN - SCOPUS:84878038512

VL - 3

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 1087

ER -