The LIM homeodomain factor Lhx2 is required for hypothalamic tanycyte specification and differentiation

Juan Salvatierra, Daniel A. Lee, Cristina Zibetti, Maria Duran-Moreno, Sooyeon Yoo, Elizabeth A. Newman, Hong Wang, Joseph L. Bedont, Jimmy De Melo, Ana L. Miranda-Angulo, Sara Gil-Perotin, Jose Manuel Garcia-Verdugo, Seth Blackshaw

Research output: Contribution to journalArticle

Abstract

Hypothalamic tanycytes, a radial glial-like ependymal cell population that expresses numerous genes selectively enriched in embryonic hypothalamic progenitors and adult neural stem cells, have recently been observed to serve as a source of adult-born neurons in the mammalian brain. The genetic mechanisms that regulate the specification and maintenance of tanycyte identity are unknown, but are critical for understanding how these cells can act as adult neural progenitor cells. We observe that LIM (Lin-11, Isl-1, Mec-3)-homeodomain gene Lhx2 is selectively expressed in hypothalamic progenitor cells and tanycytes. To test the function of Lhx2 in tanycyte development, we used an intersectional genetic strategy to conditionally delete Lhx2 in posteroventral hypothalamic neuroepithelium, both embryonically and postnatally. We observed that tanycyte development was severely disrupted when Lhx2 function was ablated during embryonic development. Lhx2-deficient tanycytes lost expression of tanycyte-specific genes, such as Rax, while also displaying ectopic expression of genes specific to cuboid ependymal cells, such as Rarres2. Ultrastructural analysis revealed that mutant tanycytes exhibited a hybrid identity, retaining radial morphology while becoming multiciliated. In contrast, postnatal loss of function of Lhx2 resulted only in loss of expression of tanycyte-specific genes. Using chromatin immunoprecipitation, we further showed that Lhx2 directly regulated expression of Rax, an essential homeodomain factor for tanycyte development. This study identifies Lhx2 as a key intrinsic regulator of tanycyte differentiation, sustaining Rax-dependent activation of tanycyte-specific genes while also inhibiting expression of ependymal cell-specific genes. These findings provide key insights into the transcriptional regulatory network specifying this still poorly characterized cell type.

Original languageEnglish (US)
Pages (from-to)16809-16820
Number of pages12
JournalJournal of Neuroscience
Volume34
Issue number50
DOIs
StatePublished - Dec 10 2014

Fingerprint

Ependymoglial Cells
Genes
Stem Cells
Adult Stem Cells
Neural Stem Cells
Gene Regulatory Networks
Chromatin Immunoprecipitation
Neuroglia
Embryonic Development
Maintenance
Neurons
Brain
Population

Keywords

  • Ependymal cells
  • Hypothalamus
  • Metabolism
  • Radial glia
  • Tanycytes
  • Transcription factor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The LIM homeodomain factor Lhx2 is required for hypothalamic tanycyte specification and differentiation. / Salvatierra, Juan; Lee, Daniel A.; Zibetti, Cristina; Duran-Moreno, Maria; Yoo, Sooyeon; Newman, Elizabeth A.; Wang, Hong; Bedont, Joseph L.; De Melo, Jimmy; Miranda-Angulo, Ana L.; Gil-Perotin, Sara; Garcia-Verdugo, Jose Manuel; Blackshaw, Seth.

In: Journal of Neuroscience, Vol. 34, No. 50, 10.12.2014, p. 16809-16820.

Research output: Contribution to journalArticle

Salvatierra, J, Lee, DA, Zibetti, C, Duran-Moreno, M, Yoo, S, Newman, EA, Wang, H, Bedont, JL, De Melo, J, Miranda-Angulo, AL, Gil-Perotin, S, Garcia-Verdugo, JM & Blackshaw, S 2014, 'The LIM homeodomain factor Lhx2 is required for hypothalamic tanycyte specification and differentiation', Journal of Neuroscience, vol. 34, no. 50, pp. 16809-16820. https://doi.org/10.1523/JNEUROSCI.1711-14.2014
Salvatierra, Juan ; Lee, Daniel A. ; Zibetti, Cristina ; Duran-Moreno, Maria ; Yoo, Sooyeon ; Newman, Elizabeth A. ; Wang, Hong ; Bedont, Joseph L. ; De Melo, Jimmy ; Miranda-Angulo, Ana L. ; Gil-Perotin, Sara ; Garcia-Verdugo, Jose Manuel ; Blackshaw, Seth. / The LIM homeodomain factor Lhx2 is required for hypothalamic tanycyte specification and differentiation. In: Journal of Neuroscience. 2014 ; Vol. 34, No. 50. pp. 16809-16820.
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