The latent human immunodeficiency virus (HIV) reservoir resides primarily in CD32 - CD4 + T Cells in Perinatally HIV-Infected Adolescents with Long-Term Virologic Suppression

Adit Dhummakupt, Lilly V. Siems, Dolly Singh, Ya Hui Chen, Thuy Anderson, Aleisha Collinson-Streng, Hao Zhang, Purvish Patel, Allison Agwu, Deborah Persaud

Research output: Contribution to journalArticlepeer-review

Abstract

Background High-level expression of the Fc3 receptor, CD32 hi, on CD4 + T cells was associated with enhanced human immunodeficiency virus (HIV) infection of the latent reservoir in a study of adults receiving antiretroviral therapy. We tested the hypothesis that CD32 was the preferential marker of the latent HIV reservoir in virally suppressed, perinatally HIV-infected adolescents. Methods The frequency of CD32 hi CD4 + T cells was determined by flow cytometry (N = 5) and the inducible HIV reservoir in both CD32 hi and CD32 - CD4 + T cells was quantified (N = 4) with a quantitative viral outgrowth assay. Viral outgrowth was measured by the standard p24 enzyme-linked immunosorbent assay and an ultrasensitive p24 assay (Simoa; Quanterix) with lower limits of quantitation. Results We found a 59.55-fold enrichment in the absolute number of infectious cells in the CD32 - population compared with CD32 hi cells. Exponential HIV replication occurred exclusively in CD32 - CD4 + T cells (mean change, 17.46 pg/mL; P =.04). Induced provirus in CD32 hi CD4 + T cells replicated to substantially lower levels, which did not increase significantly over time (mean change, 0.026 pg/mL; P =.23) and were detected only with the Simoa assay. Conclusions Our data suggests that the latent HIV reservoir resides mainly in CD32 - CD4 + T cells in virally suppressed, perinatally HIV-infected adolescents, which has implications for reservoir elimination strategies.

Original languageEnglish (US)
Pages (from-to)80-88
Number of pages9
JournalJournal of Infectious Diseases
Volume219
Issue number1
DOIs
StatePublished - Jan 1 2019

Keywords

  • CD32 HIV reservoir
  • HIV latent reservoir
  • HIV/AIDS
  • perinatal infection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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