The large-Scale isolation of bryostatin 1 from bugula neritina following current good manufacturing practices

Mary P. Koleck; Angel M. Vatakis; A. Belinda Alvarado; Pamela Andrews; L. V. Marzo; G. M. Muschik; John Roach; John T. Ross; William B. Lebherz; Marie P. Reeves; Robert M. Eberwein; Lori L. Rodgers; Robert P. Testerman; Kenneth M. Snader; Salvatore Forenza; Daniel E. Schaufelberger; John A. Beutler

doi:10.1021/np50077a004

The large-Scale isolation of bryostatin 1 from bugula neritina following current good manufacturing practices

Mary P. Koleck, Angel M. Vatakis, A. Belinda Alvarado, Pamela Andrews, L. V. Marzo, G. M. Muschik, John Roach, John T. Ross, William B. Lebherz, Marie P. Reeves, Robert M. Eberwein, Lori L. Rodgers, Robert P. Testerman, Kenneth M. Snader, Salvatore Forenza, Daniel E. Schaufelberger, John A. Beutler

Research output: Contribution to journal › Article › peer-review

124 Scopus citations

Abstract

A novel process was designed for the large-scale isolation of bryostatin 1 [1] from the bryozoan Bugula neritina L. in order to obtain multigram quantities of highly pure material for formulation studies, preclinical toxicology, and clinical trials in cancer patients. Multigram quantities of bryostatin 1 were obtained from a collection of approximately 10,000 gallons of wet animal. A phorbol dibutyrate (PDBu) receptor binding assay and hplc with photo-diode array detection were used for the design, validation, and control of the isolation process.

Original language	English (US)
Pages (from-to)	1265-1270
Number of pages	6
Journal	Journal of Natural Products
Volume	54
Issue number	5
DOIs	https://doi.org/10.1021/np50077a004
State	Published - Sep 1 1991
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

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Koleck, M. P., Vatakis, A. M., Belinda Alvarado, A., Andrews, P., Marzo, L. V., Muschik, G. M., Roach, J., Ross, J. T., Lebherz, W. B., Reeves, M. P., Eberwein, R. M., Rodgers, L. L., Testerman, R. P., Snader, K. M., Forenza, S., Schaufelberger, D. E., & Beutler, J. A. (1991). The large-Scale isolation of bryostatin 1 from bugula neritina following current good manufacturing practices. Journal of Natural Products, 54(5), 1265-1270. https://doi.org/10.1021/np50077a004

Koleck, MP, Vatakis, AM, Belinda Alvarado, A, Andrews, P, Marzo, LV, Muschik, GM, Roach, J, Ross, JT, Lebherz, WB, Reeves, MP, Eberwein, RM, Rodgers, LL, Testerman, RP, Snader, KM, Forenza, S, Schaufelberger, DE & Beutler, JA 1991, 'The large-Scale isolation of bryostatin 1 from bugula neritina following current good manufacturing practices', Journal of Natural Products, vol. 54, no. 5, pp. 1265-1270. https://doi.org/10.1021/np50077a004

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abstract = "A novel process was designed for the large-scale isolation of bryostatin 1 [1] from the bryozoan Bugula neritina L. in order to obtain multigram quantities of highly pure material for formulation studies, preclinical toxicology, and clinical trials in cancer patients. Multigram quantities of bryostatin 1 were obtained from a collection of approximately 10,000 gallons of wet animal. A phorbol dibutyrate (PDBu) receptor binding assay and hplc with photo-diode array detection were used for the design, validation, and control of the isolation process.",

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The large-Scale isolation of bryostatin 1 from bugula neritina following current good manufacturing practices

Abstract

ASJC Scopus subject areas

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