The large-Scale isolation of bryostatin 1 from bugula neritina following current good manufacturing practices

Mary P. Koleck, Angel M. Vatakis, A. Belinda Alvarado, Pamela Andrews, L. V. Marzo, G. M. Muschik, John Roach, John T. Ross, William B. Lebherz, Marie P. Reeves, Robert M. Eberwein, Lori L. Rodgers, Robert P. Testerman, Kenneth M. Snader, Salvatore Forenza, Daniel E. Schaufelberger, John A. Beutler

Research output: Contribution to journalArticlepeer-review

Abstract

A novel process was designed for the large-scale isolation of bryostatin 1 [1] from the bryozoan Bugula neritina L. in order to obtain multigram quantities of highly pure material for formulation studies, preclinical toxicology, and clinical trials in cancer patients. Multigram quantities of bryostatin 1 were obtained from a collection of approximately 10,000 gallons of wet animal. A phorbol dibutyrate (PDBu) receptor binding assay and hplc with photo-diode array detection were used for the design, validation, and control of the isolation process.

Original languageEnglish (US)
Pages (from-to)1265-1270
Number of pages6
JournalJournal of Natural Products
Volume54
Issue number5
DOIs
StatePublished - Sep 1 1991
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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