The isolation of novel mesenchymal stromal cell chemotactic factors from the conditioned medium of tumor cells

Siang Yo Lin, Jun Yang, Allen D. Everett, Charles V. Clevenger, Mythili Koneru, Pravin J. Mishra, Barton Kamen, Debabrata Banerjee, John Glod

Research output: Contribution to journalArticle

Abstract

Bone marrow-derived mesenchymal stromal cells (MSCs) localize to solid tumors. Defining the signaling mechanisms that regulate this process is important in understanding the role of MSCs in tumor growth. Using a combination of chromatography and electrospray tandem mass spectrometry we have identified novel soluble signaling molecules that induce MSC chemotaxis present in conditioned medium of the breast carcinoma cell line MDA-MB231. Previous work has employed survey strategies using ELISA assay to identify known chemokines that promote MSC chemotaxis. While these studies provide valuable insights into the intercellular signals that impact MSC behavior, many less well-described, but potentially important soluble signaling molecules could be overlooked using these methods. Through the less directed method of column chromatography we have identified novel candidate MSC chemotactic peptides. Two proteins, cyclophilin B and hepatoma-derived growth factor were then further characterized and shown to promote MSC chemotaxis.

Original languageEnglish (US)
Pages (from-to)3107-3117
Number of pages11
JournalExperimental cell research
Volume314
Issue number17
DOIs
StatePublished - Oct 15 2008

Keywords

  • Chemotaxis
  • Mesenchymal stromal cell
  • Tumor microenvironment

ASJC Scopus subject areas

  • Cell Biology

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