TY - JOUR
T1 - The Isolation of cDNAs from OATL1 at Xp11.2 Using a 480-kb YAC
AU - Geraghty, Michael T.
AU - Brody, Lawrence C.
AU - Martin, Laura S.
AU - Marble, Michael
AU - Kearns, William
AU - Pearson, Peter
AU - Monaco, Anthony P.
AU - Lehrach, Hans
AU - Valle, David
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1993/5
Y1 - 1993/5
N2 - Using an ornithine-δ-aminotransferase (OAT) cDNA, we identified five YACs that cover two nonadjacent OAT-related loci in Xp11.2-p11.3, designated OATL1 (distal) and OATL2 (proximal). Because several retinal degenerative disorders map to this region, we used YAC2 (480 kb), which covers the most distal part of OATL1, as a probe to screen a retinal cDNA library. From 8 × 104 plaques screened, we isolated 13 clones. Two were OAT cDNAs. The remaining 11 were divided into eight groups by cross-hybridization. Groups 1-4 contain cDNAs that originate from single-copy X-linked genes in YAC2. Each has an open reading frame of >500 bp and detects one or more transcripts on a Northern blot. The gene for each was sublocalized and ordered in YAC2. The cDNAs in groups 5-8 contained two or more Alu sequences, had no open reading frames, and did not detect transcripts. The cDNAs from groups 1-4 provide expressed sequence tags and identify candidate genes for the genetic disorders that map to this region.
AB - Using an ornithine-δ-aminotransferase (OAT) cDNA, we identified five YACs that cover two nonadjacent OAT-related loci in Xp11.2-p11.3, designated OATL1 (distal) and OATL2 (proximal). Because several retinal degenerative disorders map to this region, we used YAC2 (480 kb), which covers the most distal part of OATL1, as a probe to screen a retinal cDNA library. From 8 × 104 plaques screened, we isolated 13 clones. Two were OAT cDNAs. The remaining 11 were divided into eight groups by cross-hybridization. Groups 1-4 contain cDNAs that originate from single-copy X-linked genes in YAC2. Each has an open reading frame of >500 bp and detects one or more transcripts on a Northern blot. The gene for each was sublocalized and ordered in YAC2. The cDNAs in groups 5-8 contained two or more Alu sequences, had no open reading frames, and did not detect transcripts. The cDNAs from groups 1-4 provide expressed sequence tags and identify candidate genes for the genetic disorders that map to this region.
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U2 - 10.1006/geno.1993.1208
DO - 10.1006/geno.1993.1208
M3 - Article
C2 - 8314581
AN - SCOPUS:0027310955
SN - 0888-7543
VL - 16
SP - 440
EP - 446
JO - Genomics
JF - Genomics
IS - 2
ER -