The IRIDICA BAC BSI Assay: Rapid, sensitive and culture-independent identification of bacteria and Candida in blood

David Metzgar, Mark W. Frinder, Richard E. Rothman, Stephen Peterson, Karen C. Carroll, Sean X. Zhang, Gideon D. Avornu, Megan A. Rounds, Heather E. Carolan, Donna M. Toleno, David Moore, Thomas A. Hall, Christian Massire, Gregory S. Richmond, Jose R. Gutierrez, Rangarajan Sampath, David J. Ecker, Lawrence B. Blyn

Research output: Contribution to journalArticle

Abstract

Bloodstream infection (BSI) and sepsis are rising in incidence throughout the developed world. The spread of multi-drug resistant organisms presents increasing challenges to treatment. Surviving BSI is dependent on rapid and accurate identification of causal organisms, and timely application of appropriate antibiotics. Current culture-based methods used to detect and identify agents of BSI are often too slow to impact early therapy and may fail to detect relevant organisms in many positive cases. Existing methods for direct molecular detection of microbial DNA in blood are limited in either sensitivity (likely the result of small sample volumes) or in breadth of coverage, often because the PCR primers and probes used target only a few specific pathogens. There is a clear unmet need for a sensitive molecular assay capable of identifying the diverse bacteria and yeast associated with BSI directly from uncultured whole blood samples. We have developed a method of extracting DNA from larger volumes of whole blood (5 ml per sample), amplifying multiple widely conserved bacterial and fungal genes using a mismatch- and background-tolerant PCR chemistry, and identifying hundreds of diverse organisms from the amplified fragments on the basis of species-specific genetic signatures using electrospray ionization mass spectrometry (PCR/ESI-MS). We describe the analytical characteristics of the IRIDICA BAC BSI Assay and compare its pre-clinical performance to current standard-of-care methods in a collection of prospectively collected blood specimens from patients with symptoms of sepsis. The assay generated matching results in 80% of culture-positive cases (86% when common contaminants were excluded from the analysis), and twice the total number of positive detections. The described method is capable of providing organism identifications directly from uncultured blood in less than 8 hours. Disclaimer: The IRIDICA BAC BSI Assay is not available in the United States.

Original languageEnglish (US)
Article numbere0158186
JournalPloS one
Volume11
Issue number7
DOIs
StatePublished - Jul 1 2016

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Metzgar, D., Frinder, M. W., Rothman, R. E., Peterson, S., Carroll, K. C., Zhang, S. X., Avornu, G. D., Rounds, M. A., Carolan, H. E., Toleno, D. M., Moore, D., Hall, T. A., Massire, C., Richmond, G. S., Gutierrez, J. R., Sampath, R., Ecker, D. J., & Blyn, L. B. (2016). The IRIDICA BAC BSI Assay: Rapid, sensitive and culture-independent identification of bacteria and Candida in blood. PloS one, 11(7), [e0158186]. https://doi.org/10.1371/journal.pone.0158186