The involvement of SelB in the expression of cytotoxic necrotizing factor 1 in Escherichia coli

Research output: Contribution to journalArticle

Abstract

Cytotoxic necrotizing factor 1 (CNF1) plays an important role in meningitis-causing Escherichia coli. Mini-Tn5 mutagenesis of meningitis-causing E. coli revealed that transposon mutants of selA and selB genes failed to express CNF1. We subsequently showed that SelB and selenocysteine, however, are not essential for the expression of CNF1, but the deletion of 47 amino acids of SelB at its C terminus has a dominant negative effect on CNF1 expression at the translational level. Bioinformatic analysis of the mRNA of cnf1 predicted two putative selenocysteine incorporation sequence (SECIS) elements, but we failed to detect any selenocysteine in CNF1 protein. These findings suggest that SelB is involved in translational regulation of CNF1 expression but without incorporation of selenocysteine in CNF1 protein.

Original languageEnglish (US)
Pages (from-to)1934-1940
Number of pages7
JournalFEBS Letters
Volume585
Issue number12
DOIs
StatePublished - Jun 23 2011

Fingerprint

Escherichia coli
Selenocysteine
Escherichia coli Meningitis
Mutagenesis
Bioinformatics
cytotoxic necrotizing factor type 1
Computational Biology
Proteins
Genes
Amino Acids
Messenger RNA

Keywords

  • CNF1 expression
  • Escherichia coli
  • SelB
  • Selenocysteine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

The involvement of SelB in the expression of cytotoxic necrotizing factor 1 in Escherichia coli. / Yu, Hao; Kim, Kwang Sik.

In: FEBS Letters, Vol. 585, No. 12, 23.06.2011, p. 1934-1940.

Research output: Contribution to journalArticle

@article{24d9ad2ffc144bc1af1ca6c300db492f,
title = "The involvement of SelB in the expression of cytotoxic necrotizing factor 1 in Escherichia coli",
abstract = "Cytotoxic necrotizing factor 1 (CNF1) plays an important role in meningitis-causing Escherichia coli. Mini-Tn5 mutagenesis of meningitis-causing E. coli revealed that transposon mutants of selA and selB genes failed to express CNF1. We subsequently showed that SelB and selenocysteine, however, are not essential for the expression of CNF1, but the deletion of 47 amino acids of SelB at its C terminus has a dominant negative effect on CNF1 expression at the translational level. Bioinformatic analysis of the mRNA of cnf1 predicted two putative selenocysteine incorporation sequence (SECIS) elements, but we failed to detect any selenocysteine in CNF1 protein. These findings suggest that SelB is involved in translational regulation of CNF1 expression but without incorporation of selenocysteine in CNF1 protein.",
keywords = "CNF1 expression, Escherichia coli, SelB, Selenocysteine",
author = "Hao Yu and Kim, {Kwang Sik}",
year = "2011",
month = "6",
day = "23",
doi = "10.1016/j.febslet.2011.05.012",
language = "English (US)",
volume = "585",
pages = "1934--1940",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "12",

}

TY - JOUR

T1 - The involvement of SelB in the expression of cytotoxic necrotizing factor 1 in Escherichia coli

AU - Yu, Hao

AU - Kim, Kwang Sik

PY - 2011/6/23

Y1 - 2011/6/23

N2 - Cytotoxic necrotizing factor 1 (CNF1) plays an important role in meningitis-causing Escherichia coli. Mini-Tn5 mutagenesis of meningitis-causing E. coli revealed that transposon mutants of selA and selB genes failed to express CNF1. We subsequently showed that SelB and selenocysteine, however, are not essential for the expression of CNF1, but the deletion of 47 amino acids of SelB at its C terminus has a dominant negative effect on CNF1 expression at the translational level. Bioinformatic analysis of the mRNA of cnf1 predicted two putative selenocysteine incorporation sequence (SECIS) elements, but we failed to detect any selenocysteine in CNF1 protein. These findings suggest that SelB is involved in translational regulation of CNF1 expression but without incorporation of selenocysteine in CNF1 protein.

AB - Cytotoxic necrotizing factor 1 (CNF1) plays an important role in meningitis-causing Escherichia coli. Mini-Tn5 mutagenesis of meningitis-causing E. coli revealed that transposon mutants of selA and selB genes failed to express CNF1. We subsequently showed that SelB and selenocysteine, however, are not essential for the expression of CNF1, but the deletion of 47 amino acids of SelB at its C terminus has a dominant negative effect on CNF1 expression at the translational level. Bioinformatic analysis of the mRNA of cnf1 predicted two putative selenocysteine incorporation sequence (SECIS) elements, but we failed to detect any selenocysteine in CNF1 protein. These findings suggest that SelB is involved in translational regulation of CNF1 expression but without incorporation of selenocysteine in CNF1 protein.

KW - CNF1 expression

KW - Escherichia coli

KW - SelB

KW - Selenocysteine

UR - http://www.scopus.com/inward/record.url?scp=79958134831&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79958134831&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2011.05.012

DO - 10.1016/j.febslet.2011.05.012

M3 - Article

C2 - 21570972

AN - SCOPUS:79958134831

VL - 585

SP - 1934

EP - 1940

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 12

ER -