The involvement of SelB in the expression of cytotoxic necrotizing factor 1 in Escherichia coli

Research output: Contribution to journalArticle

Abstract

Cytotoxic necrotizing factor 1 (CNF1) plays an important role in meningitis-causing Escherichia coli. Mini-Tn5 mutagenesis of meningitis-causing E. coli revealed that transposon mutants of selA and selB genes failed to express CNF1. We subsequently showed that SelB and selenocysteine, however, are not essential for the expression of CNF1, but the deletion of 47 amino acids of SelB at its C terminus has a dominant negative effect on CNF1 expression at the translational level. Bioinformatic analysis of the mRNA of cnf1 predicted two putative selenocysteine incorporation sequence (SECIS) elements, but we failed to detect any selenocysteine in CNF1 protein. These findings suggest that SelB is involved in translational regulation of CNF1 expression but without incorporation of selenocysteine in CNF1 protein.

Original languageEnglish (US)
Pages (from-to)1934-1940
Number of pages7
JournalFEBS Letters
Volume585
Issue number12
DOIs
StatePublished - Jun 23 2011

Keywords

  • CNF1 expression
  • Escherichia coli
  • SelB
  • Selenocysteine

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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