The introduction of radioisotopes into RNA by methylation in vitro

Kirby D. Smith, John L. Armstrong, Brian J. McCarthy

Research output: Contribution to journalArticle

Abstract

Either 3H or 14C may be introduced at high specific radioactivity into RNA in vitro by treatment with dimethyl sulfate. The procedure is rapid and simple and no degradation of the RNA occurs. The hybridization properties of the methylated RNA compare with those of molecules randomly labeled in vivo. The method is of potential value in cases where low metabolic activity or other biological parameters preclude labeling of RNA in vivo during synthesis.

Original languageEnglish (US)
Pages (from-to)323-330
Number of pages8
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume142
Issue number2
StatePublished - Jul 18 1967
Externally publishedYes

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Radioisotopes
Methylation
RNA
RNA Stability
Radioactivity
In Vitro Techniques
dimethyl sulfate

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The introduction of radioisotopes into RNA by methylation in vitro. / Smith, Kirby D.; Armstrong, John L.; McCarthy, Brian J.

In: BBA Section Nucleic Acids And Protein Synthesis, Vol. 142, No. 2, 18.07.1967, p. 323-330.

Research output: Contribution to journalArticle

Smith, Kirby D. ; Armstrong, John L. ; McCarthy, Brian J. / The introduction of radioisotopes into RNA by methylation in vitro. In: BBA Section Nucleic Acids And Protein Synthesis. 1967 ; Vol. 142, No. 2. pp. 323-330.
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