The intravenous anesthetic propofol inhibits hypoxia-inducible factor 1 activity in an oxygen tension-dependent manner

Satoshi Takabuchi, Kiichi Hirota, Kenichiro Nishi, Seiko Oda, Tomoyuki Oda, Koh Shingu, Arimichi Takabayashi, Takehiko Adachi, Gregg L Semenza, Kazuhiko Fukuda

Research output: Contribution to journalArticle

Abstract

Hypoxia elicits a wide range of responses that occur at different organizational levels in the body. Hypoxia is not only a signal for energy conservation and metabolic change, but triggers expression of a select set of genes. The transcription factor hypoxia-inducible factor 1 (HIF-1) is now appreciated to be a master factor of the gene induction. Although knowledge on molecular mechanisms of HIF-1 activation in response to hypoxia is accumulating, the molecular mechanism of maintenance of HIF-1 activity under normoxic conditions remains to be elucidated. We demonstrate that the intravenous anesthetic propofol reversibly inhibits HIF-1 activity and the gene expression mediated by HIF-1 by blocking the synthesis of the HIF-1α subunit under 20% or 5% O2 conditions, but not under 1% O2 conditions.

Original languageEnglish (US)
Pages (from-to)434-438
Number of pages5
JournalFEBS Letters
Volume577
Issue number3
DOIs
StatePublished - Nov 19 2004

Fingerprint

Intravenous Anesthetics
Hypoxia-Inducible Factor 1
Propofol
Oxygen
Genes
Gene expression
Energy conservation
Transcription Factors
Chemical activation
Maintenance
Gene Expression
Hypoxia

Keywords

  • Hypoxia
  • Hypoxia-inducible factor 1
  • Prolyl hydroxylase
  • Propofol
  • Von Hippel-Lindau

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The intravenous anesthetic propofol inhibits hypoxia-inducible factor 1 activity in an oxygen tension-dependent manner. / Takabuchi, Satoshi; Hirota, Kiichi; Nishi, Kenichiro; Oda, Seiko; Oda, Tomoyuki; Shingu, Koh; Takabayashi, Arimichi; Adachi, Takehiko; Semenza, Gregg L; Fukuda, Kazuhiko.

In: FEBS Letters, Vol. 577, No. 3, 19.11.2004, p. 434-438.

Research output: Contribution to journalArticle

Takabuchi, S, Hirota, K, Nishi, K, Oda, S, Oda, T, Shingu, K, Takabayashi, A, Adachi, T, Semenza, GL & Fukuda, K 2004, 'The intravenous anesthetic propofol inhibits hypoxia-inducible factor 1 activity in an oxygen tension-dependent manner', FEBS Letters, vol. 577, no. 3, pp. 434-438. https://doi.org/10.1016/j.febslet.2004.10.042
Takabuchi, Satoshi ; Hirota, Kiichi ; Nishi, Kenichiro ; Oda, Seiko ; Oda, Tomoyuki ; Shingu, Koh ; Takabayashi, Arimichi ; Adachi, Takehiko ; Semenza, Gregg L ; Fukuda, Kazuhiko. / The intravenous anesthetic propofol inhibits hypoxia-inducible factor 1 activity in an oxygen tension-dependent manner. In: FEBS Letters. 2004 ; Vol. 577, No. 3. pp. 434-438.
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AU - Oda, Tomoyuki

AU - Shingu, Koh

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