The intracellular pathway of newly formed rat liver catalase

Colvin M. Redman, Dennis J. Grab, Rajender Irukulla

Research output: Contribution to journalArticlepeer-review

Abstract

[1-14C]-l-leucine was injected into the femoral vein of male rats. At various times after injection, from 3 to 120 min, the livers were removed and fractionated into various cell components. The intracellular location of radioactive catalase and albumin were compared. Radioactive catalase and albumin both appeared at 5-10 min after injection but in different intracellular locations. Catalase was first located in the soluble cytoplasmic fraction while albumin was found in the rough endoplasmic reticulum. Peak catalase radioactivity did not occur in the rough endoplasmic reticulum until 20-40 min after injection, at which time albumin was already leaving the smooth endoplasmic reticulum. Radioactive catalase did not enter the smooth endoplasmic reticulum. The catalase which sedimented with the rough endoplasmic reticulum could be separated by sucrose gradient centrifugation from microsomal vesicles which contained nascent albumin. The radioactive catalase accumulated with time into a peroxisomal fraction but some catalase of equal specific radioactivity remained in the soluble cytoplasmic fraction even at 120 min after injection.

Original languageEnglish (US)
Pages (from-to)496-501
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume152
Issue number2
DOIs
StatePublished - 1972
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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