The interaction of thrombin and nucleotides: Effects of glycolysis in human platelets

Philip D. Zieve, Morton Schmukler

Research output: Contribution to journalArticle

Abstract

Thrombin stimulated lactate formation in intact, but not disrupted, platelets, an effect inhibited by ADP and ATP. ADP and ATP stimulated lactate formation in disrupted, but not intact, platelets, an effect inhibited by thrombin. Both nucleotides altered the electrophoretic mobility of thrombin in polyacrylamide gel without affecting its molecular weight. Binding of thrombin to nucleotides could not be demonstrated by gel filtration, equilibrium dialysis, or affinity chromatography.

Original languageEnglish (US)
Pages (from-to)350-355
Number of pages6
JournalBBA - General Subjects
Volume313
Issue number2
DOIs
StatePublished - Jul 28 1973

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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