TY - JOUR
T1 - The interaction of thrombin and nucleotides
T2 - Effects of glycolysis in human platelets
AU - Zieve, Philip D.
AU - Schmukler, Morton
N1 - Funding Information:
This work was supported in part by a grant, HL-09102, from the National Institutes of Health.
PY - 1973/7/28
Y1 - 1973/7/28
N2 - Thrombin stimulated lactate formation in intact, but not disrupted, platelets, an effect inhibited by ADP and ATP. ADP and ATP stimulated lactate formation in disrupted, but not intact, platelets, an effect inhibited by thrombin. Both nucleotides altered the electrophoretic mobility of thrombin in polyacrylamide gel without affecting its molecular weight. Binding of thrombin to nucleotides could not be demonstrated by gel filtration, equilibrium dialysis, or affinity chromatography.
AB - Thrombin stimulated lactate formation in intact, but not disrupted, platelets, an effect inhibited by ADP and ATP. ADP and ATP stimulated lactate formation in disrupted, but not intact, platelets, an effect inhibited by thrombin. Both nucleotides altered the electrophoretic mobility of thrombin in polyacrylamide gel without affecting its molecular weight. Binding of thrombin to nucleotides could not be demonstrated by gel filtration, equilibrium dialysis, or affinity chromatography.
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U2 - 10.1016/0304-4165(73)90034-2
DO - 10.1016/0304-4165(73)90034-2
M3 - Article
C2 - 4741588
AN - SCOPUS:0015837607
SN - 0304-4165
VL - 313
SP - 350
EP - 355
JO - BBA - General Subjects
JF - BBA - General Subjects
IS - 2
ER -