The interaction of natural and synthetic polyanions with mammalian nuclei. II. Nuclear swelling

Richard J. Kraemer; Donald S. Coffey

doi:10.1016/0005-2787(70)90588-5

The interaction of natural and synthetic polyanions with mammalian nuclei. II. Nuclear swelling

Richard J. Kraemer, Donald S. Coffey

School of Medicine

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Various types of polyanions were tested for their effects on nuclear swelling using a new spectrophotometric technique. Heparin, dextran sulfate and polyaspartic acids (20 000-35 000 mol. wt.) caused nuclear swelling whereas polygalacturonic acid, chondroitin sulfate, hyaluronic acid, aspartic acid and polyaspartic acid (5600 mol. wt.) showed no swelling activity. The specificity of the polyanions for nuclear swelling was correlated with their effects on the template activity of nuclear DNA and chromatin during DNA synthesis.

Original language	English (US)
Pages (from-to)	568-578
Number of pages	11
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	224
Issue number	2
DOIs	https://doi.org/10.1016/0005-2787(70)90588-5
State	Published - Dec 14 1970

ASJC Scopus subject areas

Medicine(all)

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abstract = "Various types of polyanions were tested for their effects on nuclear swelling using a new spectrophotometric technique. Heparin, dextran sulfate and polyaspartic acids (20 000-35 000 mol. wt.) caused nuclear swelling whereas polygalacturonic acid, chondroitin sulfate, hyaluronic acid, aspartic acid and polyaspartic acid (5600 mol. wt.) showed no swelling activity. The specificity of the polyanions for nuclear swelling was correlated with their effects on the template activity of nuclear DNA and chromatin during DNA synthesis.",

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note = "Funding Information: This research has been aided by grant P-515 from the American Cancer Society. D.S.C. is a U.S. Public Health Service Career and Development Awardee (I-K 3-H0-38, 642 ).",

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N2 - Various types of polyanions were tested for their effects on nuclear swelling using a new spectrophotometric technique. Heparin, dextran sulfate and polyaspartic acids (20 000-35 000 mol. wt.) caused nuclear swelling whereas polygalacturonic acid, chondroitin sulfate, hyaluronic acid, aspartic acid and polyaspartic acid (5600 mol. wt.) showed no swelling activity. The specificity of the polyanions for nuclear swelling was correlated with their effects on the template activity of nuclear DNA and chromatin during DNA synthesis.

AB - Various types of polyanions were tested for their effects on nuclear swelling using a new spectrophotometric technique. Heparin, dextran sulfate and polyaspartic acids (20 000-35 000 mol. wt.) caused nuclear swelling whereas polygalacturonic acid, chondroitin sulfate, hyaluronic acid, aspartic acid and polyaspartic acid (5600 mol. wt.) showed no swelling activity. The specificity of the polyanions for nuclear swelling was correlated with their effects on the template activity of nuclear DNA and chromatin during DNA synthesis.

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The interaction of natural and synthetic polyanions with mammalian nuclei. II. Nuclear swelling

Abstract

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