The interaction between ADAM22 and 14-3-3β

Pengcheng Zhu; Yingying Sang; Rener Xu; Jing Zhao; Changben Li; Shouyuan Zhao

doi:10.1360/02yc9064

The interaction between ADAM22 and 14-3-3β

Pengcheng Zhu, Yingying Sang, Rener Xu, Jing Zhao, Changben Li, Shouyuan Zhao

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

ADAM family consists of a number of transmembrane proteins that contain a disintegrin and metalloprotease domain. ADAMs are involved in a highly diverse set of biological processes, including fertilization, neurogenesis, myogenesis and inflammatory response. The ADAM proteins have both cell adhesion and protease activities. Adam22 is highly expressed in human brain. The adam22-/- mice presented severe ataxia and died before weaning, but the function of ADAM22 is still unknown. 14-3-3 β interacting with ADAM22 was detected by using yeast two-hybrid assay. The specificity of interaction between ADAM22 and 14-3-3β was proved by in vitro binding assay and immunoprecipitation. The major 14-3-3β binding site was located in the last 28 amino acid residues of ADAM22 cytoplasmic tail. Protein 14-3-3β is abundant and plays an important role in mediating cell diffusion, migration and cell cycle control. The interaction of ADAM22 and 14-3-3β suggests that the ADAM22 may play a crucial role in neural function and development.

Original language	English (US)
Pages (from-to)	577-582
Number of pages	6
Journal	Science in China, Series C: Life Sciences
Volume	45
Issue number	6
DOIs	https://doi.org/10.1360/02yc9064
State	Published - 2002
Externally published	Yes

Keywords

14-3-3β
ADAM22
Cell adhesion
Immunoprecipitation
Yeast two-hybrid assay

ASJC Scopus subject areas

Renewable Energy, Sustainability and the Environment
Modeling and Simulation
General Biochemistry, Genetics and Molecular Biology
Fuel Technology
General Environmental Science
Energy Engineering and Power Technology
General Agricultural and Biological Sciences
General Energy
Management, Monitoring, Policy and Law

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title = "The interaction between ADAM22 and 14-3-3β",

abstract = "ADAM family consists of a number of transmembrane proteins that contain a disintegrin and metalloprotease domain. ADAMs are involved in a highly diverse set of biological processes, including fertilization, neurogenesis, myogenesis and inflammatory response. The ADAM proteins have both cell adhesion and protease activities. Adam22 is highly expressed in human brain. The adam22-/- mice presented severe ataxia and died before weaning, but the function of ADAM22 is still unknown. 14-3-3 β interacting with ADAM22 was detected by using yeast two-hybrid assay. The specificity of interaction between ADAM22 and 14-3-3β was proved by in vitro binding assay and immunoprecipitation. The major 14-3-3β binding site was located in the last 28 amino acid residues of ADAM22 cytoplasmic tail. Protein 14-3-3β is abundant and plays an important role in mediating cell diffusion, migration and cell cycle control. The interaction of ADAM22 and 14-3-3β suggests that the ADAM22 may play a crucial role in neural function and development.",

keywords = "14-3-3β, ADAM22, Cell adhesion, Immunoprecipitation, Yeast two-hybrid assay",

author = "Pengcheng Zhu and Yingying Sang and Rener Xu and Jing Zhao and Changben Li and Shouyuan Zhao",

note = "Funding Information: Acknowledgements We thank Dr. Wang Tongwen for the materials and technical advice, and Dr. Feng Libing and Ph.D. candidate Xu Huanbing for technical assistance. This work was supported by the National Natural Science Foundation of China (Grant No. 30170866).",

year = "2002",

doi = "10.1360/02yc9064",

language = "English (US)",

volume = "45",

pages = "577--582",

journal = "Science in China, Series C: Life Sciences",

issn = "1006-9305",

publisher = "Science in China Press",

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TY - JOUR

T1 - The interaction between ADAM22 and 14-3-3β

AU - Zhu, Pengcheng

AU - Sang, Yingying

AU - Xu, Rener

AU - Zhao, Jing

AU - Li, Changben

AU - Zhao, Shouyuan

N1 - Funding Information: Acknowledgements We thank Dr. Wang Tongwen for the materials and technical advice, and Dr. Feng Libing and Ph.D. candidate Xu Huanbing for technical assistance. This work was supported by the National Natural Science Foundation of China (Grant No. 30170866).

PY - 2002

Y1 - 2002

N2 - ADAM family consists of a number of transmembrane proteins that contain a disintegrin and metalloprotease domain. ADAMs are involved in a highly diverse set of biological processes, including fertilization, neurogenesis, myogenesis and inflammatory response. The ADAM proteins have both cell adhesion and protease activities. Adam22 is highly expressed in human brain. The adam22-/- mice presented severe ataxia and died before weaning, but the function of ADAM22 is still unknown. 14-3-3 β interacting with ADAM22 was detected by using yeast two-hybrid assay. The specificity of interaction between ADAM22 and 14-3-3β was proved by in vitro binding assay and immunoprecipitation. The major 14-3-3β binding site was located in the last 28 amino acid residues of ADAM22 cytoplasmic tail. Protein 14-3-3β is abundant and plays an important role in mediating cell diffusion, migration and cell cycle control. The interaction of ADAM22 and 14-3-3β suggests that the ADAM22 may play a crucial role in neural function and development.

AB - ADAM family consists of a number of transmembrane proteins that contain a disintegrin and metalloprotease domain. ADAMs are involved in a highly diverse set of biological processes, including fertilization, neurogenesis, myogenesis and inflammatory response. The ADAM proteins have both cell adhesion and protease activities. Adam22 is highly expressed in human brain. The adam22-/- mice presented severe ataxia and died before weaning, but the function of ADAM22 is still unknown. 14-3-3 β interacting with ADAM22 was detected by using yeast two-hybrid assay. The specificity of interaction between ADAM22 and 14-3-3β was proved by in vitro binding assay and immunoprecipitation. The major 14-3-3β binding site was located in the last 28 amino acid residues of ADAM22 cytoplasmic tail. Protein 14-3-3β is abundant and plays an important role in mediating cell diffusion, migration and cell cycle control. The interaction of ADAM22 and 14-3-3β suggests that the ADAM22 may play a crucial role in neural function and development.

KW - 14-3-3β

KW - ADAM22

KW - Cell adhesion

KW - Immunoprecipitation

KW - Yeast two-hybrid assay

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The interaction between ADAM22 and 14-3-3β

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Keywords

ASJC Scopus subject areas

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