The interaction between ADAM22 and 14-3-3β

Pengcheng Zhu, Yingying Sang, Rener Xu, Jing Zhao, Changben Li, Shouyuan Zhao

Research output: Contribution to journalArticle

Abstract

ADAM family consists of a number of transmembrane proteins that contain a disintegrin and metalloprotease domain. ADAMs are involved in a highly diverse set of biological processes, including fertilization, neurogenesis, myogenesis and inflammatory response. The ADAM proteins have both cell adhesion and protease activities. Adam22 is highly expressed in human brain. The adam22-/- mice presented severe ataxia and died before weaning, but the function of ADAM22 is still unknown. 14-3-3 β interacting with ADAM22 was detected by using yeast two-hybrid assay. The specificity of interaction between ADAM22 and 14-3-3β was proved by in vitro binding assay and immunoprecipitation. The major 14-3-3β binding site was located in the last 28 amino acid residues of ADAM22 cytoplasmic tail. Protein 14-3-3β is abundant and plays an important role in mediating cell diffusion, migration and cell cycle control. The interaction of ADAM22 and 14-3-3β suggests that the ADAM22 may play a crucial role in neural function and development.

Original languageEnglish (US)
Pages (from-to)577-582
Number of pages6
JournalScience in China, Series C: Life Sciences
Volume45
Issue number6
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

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Keywords

  • 14-3-3β
  • ADAM22
  • Cell adhesion
  • Immunoprecipitation
  • Yeast two-hybrid assay

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

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