The inositol hexakisphosphate kinase family. Catalytic flexibility and function in yeast vacuole biogenesis

A. Saiardi, J. J. Caffrey, Solomon H Snyder, S. B. Shears

Research output: Contribution to journalArticle

Abstract

Saiardi et al. (Saiardi, A., Erdjument-Bromage, H., Snowman, A., Tempst, P., and Snyder, S. H. (1999) Curr. Biol. 9, 1323-1326) previously described the cloning of a kinase from yeast and two kinases from mammals (types 1 and 2), which phosphorylate inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate, a 'high energy' candidate regulator of cellular trafficking. We have now studied the significance of InsP6 kinase activity in Saccharomyces cerevisiae by disrupting the kinase gene. These ip6kΔ cells grew more slowly, their levels of diphosphoinositol polyphosphates were 60-80% lower than wild-type cells, and the cells contained abnormally small and fragmented vacuoles. Novel activities of the mammalian and yeast InsP6 kinases were identified; inositol pentakisphosphate (InsP5) was phosphorylated to diphosphoinositol tetrakisphosphate (PP-InsP4), which was further metabolized to a novel compound, tentatively identified as bisdiphosphoinositol trisphosphate. The latter is a new substrate for human diphosphoinositol polyphosphate phosphohydrolase. Kinetic parameters for the mammalian type 1 kinase indicate that InsP5 (K(m) = 1.2μM) and InsP6 (K(m) = 6.7 μM) compete for phosphorylation in vivo. This is the first time a PP-InsP4 synthase has been identified. The mammalian type 2 kinase and the yeast kinase are more specialized for the phosphorylation of InsP6. Synthesis of the diphosphorylated inositol phosphates is thus revealed to be more complex and interdependent than previously envisaged.

Original languageEnglish (US)
Pages (from-to)24686-24692
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number32
DOIs
StatePublished - Aug 11 2000
Externally publishedYes

Fingerprint

Vacuoles
Yeast
Phosphotransferases
Yeasts
Phosphorylation
Polyphosphates
Phytic Acid
Inositol Phosphates
Mammals
inositol hexakisphosphate kinase
Cloning
Saccharomyces cerevisiae
Organism Cloning
Kinetic parameters
Genes
Substrates
inositol pentaphosphate

ASJC Scopus subject areas

  • Biochemistry

Cite this

The inositol hexakisphosphate kinase family. Catalytic flexibility and function in yeast vacuole biogenesis. / Saiardi, A.; Caffrey, J. J.; Snyder, Solomon H; Shears, S. B.

In: Journal of Biological Chemistry, Vol. 275, No. 32, 11.08.2000, p. 24686-24692.

Research output: Contribution to journalArticle

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