@article{64037abe53f94b6487e9ae1464d93f2c,
title = "The innate immune sensor IFI16 recognizes foreign DNA in the nucleus by scanning along the duplex",
abstract = "The ability to recognize foreign double-stranded (ds)DNA of pathogenic origin in the intracellular environment is an essential defense mechanism of the human innate immune system. However, the molecular mechanisms underlying distinction between foreign DNA and host genomic material inside the nucleus are not understood. By combining biochemical assays and single-molecule techniques, we show that the nuclear innate immune sensor IFI16 onedimensionally tracks long stretches of exposed foreign dsDNA to assemble into supramolecular signaling platforms. We also demonstrate that nucleosomes represent barriers that prevent IFI16 from targeting host DNA by directly interfering with these one-dimensional movements. This unique scanning-assisted assembly mechanism allows IFI16 to distinguish friend from foe and assemble into oligomers efficiently and selectively on foreign DNA.",
author = "Stratmann, {Sarah A.} and Morrone, {Seamus R.} and {van Oijen}, {Antoine M.} and Jungsan Sohn",
note = "Funding Information: We thank Drs. Cynthia Wolberger and Gregory Bowman for their assistance in generating di-nucleo- somes, Michiel Punter and Dr. Victor Krasnikov for advice with data analysis and microscopy, and Dr. Karl Duderstadt and Dr. Bastian Niebel for a critical reading of the manuscript. JS acknowledges support from the Jerome L. Greene Foundation and AvO from the European Research Council (ERC Starting 281098) and the Australian Research Council (FL140100027). Funding Information: We thank Drs. Cynthia Wolberger and Gregory Bowman for their assistance in generating di-nucleosomes, Michiel Punter and Dr. Victor Krasnikov for advice with data analysis and microscopy, and Dr. Karl Duderstadt and Dr. Bastian Niebel for a critical reading of the manuscript. JS acknowledges support from the Jerome L. Greene Foundation and AvO from the European Research Council (ERC Starting 281098) and the Australian Research Council (FL140100027). Publisher Copyright: {\textcopyright} Stratmann et al.",
year = "2015",
month = dec,
day = "16",
doi = "10.7554/eLife.11721",
language = "English (US)",
volume = "4",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
number = "DECEMBER2015",
}