The inhibitory effect of ambroxol on hypochlorous acid-induced tissue damage and respiratory burst of phagocytic cells

Yong Cho, Yoon Young Jang, Eun Sook Han, Chung Soo Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Ambroxol (100 μM and 1 mM) and the thiols (all 1 mM), glutathione, tiopronin and cysteine, significantly attenuated the myeloperoxidase, H2O2 and Cl- system-caused destruction of α1-antiproteinase and the HOCl-induced destruction of collagen, whereas they did not affect the elastase-induced destruction of collagen. Glutathione, tiopronin and cysteine almost completely decomposed both HOCl and H2O2, while ambroxol up to 1 mM did not show a scavenging action on H2O2. Ambroxol (1 to 100 μM) and 1 mM thiol compounds markedly inhibited the HOCl-induced alteration of elastase activity. Thiol compounds significantly attenuated the HOCl production caused by degraded immunoglobulin G-activated neutrophils. Ambroxol depressed superoxide and H2O2 production induced by degraded immunoglobulin G-activated neutrophils and by lipopolysaccharide-activated alveolar macrophages in a dose-dependent manner. The results show that ambroxol may interfere with oxidative tissue damage and decrease proteolytic tissue destruction by attenuation of oxidative stress-induced inactivation of α1-antiproteinase through both decomposition of HOCl and inhibition of the respiratory burst in phagocytic cells. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)83-91
Number of pages9
JournalEuropean Journal of Pharmacology
Volume383
Issue number1
DOIs
StatePublished - Oct 21 1999
Externally publishedYes

Keywords

  • Ambroxol
  • Hypochlorous acid
  • Respiratory burst
  • α-Antiproteinase destruction

ASJC Scopus subject areas

  • Pharmacology

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