TY - JOUR
T1 - The inhibitory effect of ambroxol on hypochlorous acid-induced tissue damage and respiratory burst of phagocytic cells
AU - Cho, Yong
AU - Jang, Yoon Young
AU - Han, Eun Sook
AU - Lee, Chung Soo
PY - 1999/10/21
Y1 - 1999/10/21
N2 - Ambroxol (100 μM and 1 mM) and the thiols (all 1 mM), glutathione, tiopronin and cysteine, significantly attenuated the myeloperoxidase, H2O2 and Cl- system-caused destruction of α1-antiproteinase and the HOCl-induced destruction of collagen, whereas they did not affect the elastase-induced destruction of collagen. Glutathione, tiopronin and cysteine almost completely decomposed both HOCl and H2O2, while ambroxol up to 1 mM did not show a scavenging action on H2O2. Ambroxol (1 to 100 μM) and 1 mM thiol compounds markedly inhibited the HOCl-induced alteration of elastase activity. Thiol compounds significantly attenuated the HOCl production caused by degraded immunoglobulin G-activated neutrophils. Ambroxol depressed superoxide and H2O2 production induced by degraded immunoglobulin G-activated neutrophils and by lipopolysaccharide-activated alveolar macrophages in a dose-dependent manner. The results show that ambroxol may interfere with oxidative tissue damage and decrease proteolytic tissue destruction by attenuation of oxidative stress-induced inactivation of α1-antiproteinase through both decomposition of HOCl and inhibition of the respiratory burst in phagocytic cells. Copyright (C) 1999 Elsevier Science B.V.
AB - Ambroxol (100 μM and 1 mM) and the thiols (all 1 mM), glutathione, tiopronin and cysteine, significantly attenuated the myeloperoxidase, H2O2 and Cl- system-caused destruction of α1-antiproteinase and the HOCl-induced destruction of collagen, whereas they did not affect the elastase-induced destruction of collagen. Glutathione, tiopronin and cysteine almost completely decomposed both HOCl and H2O2, while ambroxol up to 1 mM did not show a scavenging action on H2O2. Ambroxol (1 to 100 μM) and 1 mM thiol compounds markedly inhibited the HOCl-induced alteration of elastase activity. Thiol compounds significantly attenuated the HOCl production caused by degraded immunoglobulin G-activated neutrophils. Ambroxol depressed superoxide and H2O2 production induced by degraded immunoglobulin G-activated neutrophils and by lipopolysaccharide-activated alveolar macrophages in a dose-dependent manner. The results show that ambroxol may interfere with oxidative tissue damage and decrease proteolytic tissue destruction by attenuation of oxidative stress-induced inactivation of α1-antiproteinase through both decomposition of HOCl and inhibition of the respiratory burst in phagocytic cells. Copyright (C) 1999 Elsevier Science B.V.
KW - Ambroxol
KW - Hypochlorous acid
KW - Respiratory burst
KW - α-Antiproteinase destruction
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U2 - 10.1016/S0014-2999(99)00585-3
DO - 10.1016/S0014-2999(99)00585-3
M3 - Article
C2 - 10556685
AN - SCOPUS:0033592587
SN - 0014-2999
VL - 383
SP - 83
EP - 91
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 1
ER -