TY - JOUR
T1 - The Influenza A virus M2 protein trans-complementation system offers a set of tools for the undergraduate virology laboratory
AU - Grantham, Michael L.
AU - McCown, Matthew F.
AU - Pekosz, Andrew
N1 - Publisher Copyright:
©2019 Author(s).
PY - 2019
Y1 - 2019
N2 - An authentic, hands-on experience in the laboratory is an important part of any undergraduate biology course. However, there are a limited number of mammalian virus systems that students can work with safely in an undergraduate teaching laboratory. For many systems, the risk to the students is too high. The influenza A virus M2 protein trans-complementation system bridges this gap. This system consists of a virus with mutations that prevent the expression of the essential M2 protein; therefore this virus can only replicate in a cell line that provides M2 in trans. Here, we describe the use of this system to carry out hemagglutination, real-time reverse transcriptase PCR, 50% tissue culture infectious dose, and plaque assays in an undergraduate lab setting.
AB - An authentic, hands-on experience in the laboratory is an important part of any undergraduate biology course. However, there are a limited number of mammalian virus systems that students can work with safely in an undergraduate teaching laboratory. For many systems, the risk to the students is too high. The influenza A virus M2 protein trans-complementation system bridges this gap. This system consists of a virus with mutations that prevent the expression of the essential M2 protein; therefore this virus can only replicate in a cell line that provides M2 in trans. Here, we describe the use of this system to carry out hemagglutination, real-time reverse transcriptase PCR, 50% tissue culture infectious dose, and plaque assays in an undergraduate lab setting.
UR - http://www.scopus.com/inward/record.url?scp=85070737792&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85070737792&partnerID=8YFLogxK
U2 - 10.1128/jmbe.v20i1.1667
DO - 10.1128/jmbe.v20i1.1667
M3 - Article
C2 - 31160927
AN - SCOPUS:85070737792
SN - 1935-7877
VL - 20
JO - Journal of Microbiology and Biology Education
JF - Journal of Microbiology and Biology Education
IS - 1
ER -