The Influenza A virus M2 protein trans-complementation system offers a set of tools for the undergraduate virology laboratory

Michael L. Grantham, Matthew F. McCown, Andrew Pekosz

Research output: Contribution to journalArticle

Abstract

An authentic, hands-on experience in the laboratory is an important part of any undergraduate biology course. However, there are a limited number of mammalian virus systems that students can work with safely in an undergraduate teaching laboratory. For many systems, the risk to the students is too high. The influenza A virus M2 protein trans-complementation system bridges this gap. This system consists of a virus with mutations that prevent the expression of the essential M2 protein; therefore this virus can only replicate in a cell line that provides M2 in trans. Here, we describe the use of this system to carry out hemagglutination, real-time reverse transcriptase PCR, 50% tissue culture infectious dose, and plaque assays in an undergraduate lab setting.

Original languageEnglish (US)
JournalJournal of Microbiology and Biology Education
Volume20
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

virology
Virology
college students
Viruses
Influenza A virus
contagious disease
viruses
students
Students
Tissue culture
proteins
RNA-Directed DNA Polymerase
Hemagglutination
hemagglutination
Reverse Transcriptase Polymerase Chain Reaction
tissue culture
Real-Time Polymerase Chain Reaction
Assays
Teaching
reverse transcriptase polymerase chain reaction

ASJC Scopus subject areas

  • Education
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

The Influenza A virus M2 protein trans-complementation system offers a set of tools for the undergraduate virology laboratory. / Grantham, Michael L.; McCown, Matthew F.; Pekosz, Andrew.

In: Journal of Microbiology and Biology Education, Vol. 20, No. 1, 01.01.2019.

Research output: Contribution to journalArticle

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