The influences of reproductive status and acute stress on the levels of phosphorylated delta opioid receptor immunoreactivity in rat hippocampus

Suzanne R. Burstein, Tanya McDonald, Diane A. Lane, Margarete G. Knudsen, Virginia M. Pickel, Bruce S. McEwen, Elizabeth M. Waters, Teresa A. Milner

Research output: Contribution to journalArticle

Abstract

In the hippocampus, ovarian hormones and sex can alter the trafficking of delta opioid receptors (DORs) and the proportion of DORs that colocalize with the stress hormone, corticotropin releasing factor. Here, we assessed the effects of acute immobilization stress (AIS) and sex on the phosphorylation of DORs in the rat hippocampus. We first localized an antibody to phosphorylated DOR (pDOR) at the SER363 carboxy-terminal residue, and demonstrated its response to an opioid agonist. By light microscopy, pDOR-immunoreactivity (ir) was located predominantly in CA2/CA3a pyramidal cell apical dendrites and in interneurons in CA1-3 stratum oriens and the dentate hilus. By electron microscopy, pDOR-ir primarily was located in somata and dendrites, associated with endomembranes, or in dendritic spines. pDOR-ir was less frequently found in mossy fibers terminals. Quantitative light microscopy revealed a significant increase in pDOR-ir in the CA2/CA3a region of male rats 1 h following an injection of the opioid agonist morphine (20 mg/kg, I.P). To look at the effects of stress on pDOR, we compared pDOR-ir in males and cycling females after AIS. The level of pDOR-ir in stratum radiatum of CA2/CA3a was increased in control estrus (elevated estrogen and progesterone) females compared to proestrus and diestrus females and males. However, immediately following 30 min of AIS, no significant differences in pDOR levels were seen across estrous cycle phase or sex. These findings suggest that hippocampal levels of phosphorylated DORs vary with estrous cycle phase and that acute stress may dampen the differential effects of hormones on DOR activation in females.

Original languageEnglish (US)
Pages (from-to)71-81
Number of pages11
JournalBrain Research
Volume1518
DOIs
StatePublished - Jun 26 2013
Externally publishedYes

Fingerprint

delta Opioid Receptor
Hippocampus
Immobilization
Estrous Cycle
Dendrites
Opioid Analgesics
Hippocampal CA2 Region
Microscopy
Hormones
Diestrus
Light
Proestrus
Dendritic Spines
Pyramidal Cells
Corticotropin-Releasing Hormone
Estrus
Gonadal Steroid Hormones
Carisoprodol
Interneurons
Morphine

Keywords

  • CA2
  • Estrogens
  • Mossy fiber pathway
  • Opioids
  • Pyramidal cells
  • region of the hippocampus
  • Sex differences

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Developmental Biology
  • Clinical Neurology

Cite this

The influences of reproductive status and acute stress on the levels of phosphorylated delta opioid receptor immunoreactivity in rat hippocampus. / Burstein, Suzanne R.; McDonald, Tanya; Lane, Diane A.; Knudsen, Margarete G.; Pickel, Virginia M.; McEwen, Bruce S.; Waters, Elizabeth M.; Milner, Teresa A.

In: Brain Research, Vol. 1518, 26.06.2013, p. 71-81.

Research output: Contribution to journalArticle

Burstein, Suzanne R. ; McDonald, Tanya ; Lane, Diane A. ; Knudsen, Margarete G. ; Pickel, Virginia M. ; McEwen, Bruce S. ; Waters, Elizabeth M. ; Milner, Teresa A. / The influences of reproductive status and acute stress on the levels of phosphorylated delta opioid receptor immunoreactivity in rat hippocampus. In: Brain Research. 2013 ; Vol. 1518. pp. 71-81.
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AU - Waters, Elizabeth M.

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