The influence of retinoids on cultivated human keratinocytes

Joseph McGuire, Neal S Fedarko, Erik Johanssen, Jane LaVigne, Gary Lyons, Leonard Milstone, Michael Osber

Research output: Contribution to journalArticle

Abstract

Cultured keratinocytes afford an excellent opportunity to study the influence of retinoids on the behavior of a stratified squamous epithelium and the interaction of keratinocytes with substrate. We have found that all-trans-retinoic acid retards the formation of colonies, does not influence attachment, and causes increased shedding of cells from the cultures. Retinoids do not influence the relative abundance of the keratin polypeptides. Our observations are on human neonatal foreskin-derived keratinocytes grown in Dulbecco's modified Eagle medium containing 20% fetal bovine serum. Because fetal bovine serum contains vitamin A, our findings represent differences between low and high levels of vitamin A.

Original languageEnglish (US)
Pages (from-to)630-639
Number of pages10
JournalJournal of the American Academy of Dermatology
Volume6
Issue number4
DOIs
StatePublished - Jan 1 1982
Externally publishedYes

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Retinoids
Keratinocytes
Vitamin A
Foreskin
Eagles
Keratins
Tretinoin
Serum
Epithelium
Cell Culture Techniques
Peptides

ASJC Scopus subject areas

  • Dermatology

Cite this

The influence of retinoids on cultivated human keratinocytes. / McGuire, Joseph; Fedarko, Neal S; Johanssen, Erik; LaVigne, Jane; Lyons, Gary; Milstone, Leonard; Osber, Michael.

In: Journal of the American Academy of Dermatology, Vol. 6, No. 4, 01.01.1982, p. 630-639.

Research output: Contribution to journalArticle

McGuire, J, Fedarko, NS, Johanssen, E, LaVigne, J, Lyons, G, Milstone, L & Osber, M 1982, 'The influence of retinoids on cultivated human keratinocytes', Journal of the American Academy of Dermatology, vol. 6, no. 4, pp. 630-639. https://doi.org/10.1016/S0190-9622(82)70052-0
McGuire, Joseph ; Fedarko, Neal S ; Johanssen, Erik ; LaVigne, Jane ; Lyons, Gary ; Milstone, Leonard ; Osber, Michael. / The influence of retinoids on cultivated human keratinocytes. In: Journal of the American Academy of Dermatology. 1982 ; Vol. 6, No. 4. pp. 630-639.
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