The influence of pulsed low-intensity ultrasound on matrix production of chondrocytes at different stages of differentiation

An explant study

Zi Jun Zhang, James Huckle, Clair Ann Francomano, Richard G S Spencer

Research output: Contribution to journalArticle

Abstract

The proximal and distal parts of sterna of chick embryos represent cartilage undergoing endochondral ossification and hyaline cartilage, respectively. Cartilage explants from both regions were exposed for 20 min to pulsed low-intensity ultrasound (PLIUS) with an intensity of 30 mW·cm-2 (spatial average-temporal average) at a frequency of 1.5 MHz, with a pulse burst frequency of 1 kHz and burst duration of 200 μs. Histological and immunohistochemical analysis was performed on days 1, 3, 5 and 7 after treatment. An anabolic effect of PLIUS on matrix production was shown by an increase of up to 10% to 20% in quantitative immunohistochemical staining for type II collagen and aggrecan in the two parts of the sternum. PLIUS also increased type X collagen staining by up to 10% in certain regions of the proximal part of the sternum. Staining for type X collagen was negative in the distal part of the sternum in both PLIUS and control groups. These results suggest that PLIUS may stimulate bone formation by increasing hypertrophy of chondrocytes directed to terminal differentiation. However, PLIUS did not induce hypertrophy in hyaline cartilage; moreover, increased matrix synthesis indicates a potential role in cartilage repair.

Original languageEnglish (US)
Pages (from-to)1547-1553
Number of pages7
JournalUltrasound in Medicine and Biology
Volume28
Issue number11-12
DOIs
StatePublished - Nov 2002

Fingerprint

Chondrocytes
sternum
cartilage
Sternum
matrices
Collagen Type X
staining
collagens
Cartilage
Hyaline Cartilage
Staining and Labeling
Osteogenesis
Hypertrophy
bursts
Aggrecans
Anabolic Agents
Collagen Type II
osteogenesis
Chick Embryo
embryos

Keywords

  • Cartilage
  • Chondrocyte
  • Ossification
  • Ultrasound

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

The influence of pulsed low-intensity ultrasound on matrix production of chondrocytes at different stages of differentiation : An explant study. / Zhang, Zi Jun; Huckle, James; Francomano, Clair Ann; Spencer, Richard G S.

In: Ultrasound in Medicine and Biology, Vol. 28, No. 11-12, 11.2002, p. 1547-1553.

Research output: Contribution to journalArticle

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