The induction of organ-specific antibodies during the graft-vs.-host reaction

R. C. Kuppers, T. Suiter, E. Gleichmann, N. R. Rose

Research output: Contribution to journalArticle

Abstract

During the parent (P) into F1 hybrid graft-vs.-host reaction (GVHR), nuclear, leukocyte and erythrocyte autoantibodies are commonly seen. The specificity of these autoantibodies is reminiscent of those found in systemic lupus erythematosus (SLE) patients and SLE-prone mice. Organ-specific antibodies, however, including thyroglobulin (Tg) antibodies do not arise spontaneously. There have been conflicting reports about the ability of exogenous Tg to induce an anti-Tg response during the GVHR. We have re-examined this question in greater detail. Using the murine P→F1 GVHR system, the results of this work demonstrate that mouse thyroglobulin (MTg)-specific antibodies can be induced during a GVHR. However, mice must both be undergoing a GVHR, and have received exogenous MTg. The highest autoantibody response occurs if mice are injected with mouse thyroid extract or purified MTg at the time of P→T1 cell transfer. The anti-MTg response is MTg dose dependent. The ability to induce anti-MTg antibody was not major histocompatibility complex restricted, for both the DBA/2→B6D2F1 (low responder H-2 haplotypes to MTg), and AKR or DBA/2→AKD2F1 (high/low responder→high responder haplotype ) GVHR gave similar responses. The anti-MTg titers peaked between days 7-10 and declined thereafter. In contrast, antibodies to dsDNA were not present at this early time, but developed after several weeks. We conclude that organ-specific autoantibodies can be induced during a GVHR if the appropriate antigen(s) are presented near the time of GVHR induction.

Original languageEnglish (US)
Pages (from-to)161-166
Number of pages6
JournalEuropean Journal of Immunology
Volume18
Issue number1
StatePublished - 1988

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Thyroglobulin
Transplants
Antibodies
Autoantibodies
Systemic Lupus Erythematosus
Haplotypes
Inbred AKR Mouse
Inbred DBA Mouse
Major Histocompatibility Complex
Thyroid Gland
Leukocytes
Erythrocytes
Antigens
anti-thyroglobulin

ASJC Scopus subject areas

  • Immunology

Cite this

Kuppers, R. C., Suiter, T., Gleichmann, E., & Rose, N. R. (1988). The induction of organ-specific antibodies during the graft-vs.-host reaction. European Journal of Immunology, 18(1), 161-166.

The induction of organ-specific antibodies during the graft-vs.-host reaction. / Kuppers, R. C.; Suiter, T.; Gleichmann, E.; Rose, N. R.

In: European Journal of Immunology, Vol. 18, No. 1, 1988, p. 161-166.

Research output: Contribution to journalArticle

Kuppers, RC, Suiter, T, Gleichmann, E & Rose, NR 1988, 'The induction of organ-specific antibodies during the graft-vs.-host reaction', European Journal of Immunology, vol. 18, no. 1, pp. 161-166.
Kuppers, R. C. ; Suiter, T. ; Gleichmann, E. ; Rose, N. R. / The induction of organ-specific antibodies during the graft-vs.-host reaction. In: European Journal of Immunology. 1988 ; Vol. 18, No. 1. pp. 161-166.
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