The increase in mitochondrial association with actin precedes Bax translocation in apoptosis

Ho Lam Tang, Anh Huy Phan Le, Hong Lok Lung

Research output: Contribution to journalArticle

Abstract

Accumulating evidence indicates the potential role of actin cytoskeleton in facilitating the mitochondrial recruitment of various pro-apoptotic proteins from the cytosol to initiate apoptosis. In the present paper, we report the observation of the increase in mitochondrial association of actin in early apoptosis. Using cell fractionation and Western blot analysis, we found that mitochondrial accumulation of β-actin occurred before the mitochondrial insertion of Bax and release of cytochrome c in apoptosis. The mitochondrial accumulation of β-actin was observed with various apoptotic stimuli in various cell lines, suggesting that this is a general apoptotic phenomenon in mammalian systems. Using fluorescence microscopy, we have shown that an apoptotic induction triggered the reorganization of the F-actin (filamentous actin) network with an increase in the association with mitochondria, which was observed before mitochondrial fission and nuclear condensation. Perhaps actin could contribute to the initiation of apoptosis by enabling cytosolic pro-apoptotic proteins to be carried to mitochondria by the cytoskeleton-driven trafficking system.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalBiochemical Journal
Volume396
Issue number1
DOIs
StatePublished - May 15 2006

Keywords

  • Actin
  • Apoptosis
  • Bax
  • Cytoskeleton
  • Mitochondrion
  • Translocation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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