TY - JOUR
T1 - The in vivo stimulation of mannose incorporation into mannosylretinylphosphate, dolichylmannosylphosphate, and specific glycopeptides of rat liver by high doses of retinylpalmitate
AU - Hassell, J. R.
AU - Silverman-Jones, C. S.
AU - De Luca, L. M.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1978
Y1 - 1978
N2 - Excess vitamin A stimulated the incorporation of mannose into rat liver mannosylretinylphosphate (MRP), dolichylmannosylphosphate (DMP), and into glycoproteins by over 200% during a 20-min labeling period. The glycoproteins were digested with pronase and separated into three components by molecular sieve chromatography. The stimulation of mannose incorporation was greatest in the Peak II glycopeptide (M(r) = 6500). In contrast, the incorporation of galactose into glycolipids or glycopeptides was not altered by a vitamin A treatment. Analysis of the glycopeptide stimulated by vitamin A treatment showed it to contain mannose, glucose, galactose, and glucosamine in the respective molar ratios of 7:10:17:1 and to be rich in glutamic acid, serine, glycine, aspartic acid and threonine. The results suggest that excess vitamin A stimulates the incorporation of mannose into glycoproteins by enhancing the synthesis of lipid intermediates involved in specific mannosyl transfer reactions.
AB - Excess vitamin A stimulated the incorporation of mannose into rat liver mannosylretinylphosphate (MRP), dolichylmannosylphosphate (DMP), and into glycoproteins by over 200% during a 20-min labeling period. The glycoproteins were digested with pronase and separated into three components by molecular sieve chromatography. The stimulation of mannose incorporation was greatest in the Peak II glycopeptide (M(r) = 6500). In contrast, the incorporation of galactose into glycolipids or glycopeptides was not altered by a vitamin A treatment. Analysis of the glycopeptide stimulated by vitamin A treatment showed it to contain mannose, glucose, galactose, and glucosamine in the respective molar ratios of 7:10:17:1 and to be rich in glutamic acid, serine, glycine, aspartic acid and threonine. The results suggest that excess vitamin A stimulates the incorporation of mannose into glycoproteins by enhancing the synthesis of lipid intermediates involved in specific mannosyl transfer reactions.
UR - http://www.scopus.com/inward/record.url?scp=0018134771&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018134771&partnerID=8YFLogxK
M3 - Article
C2 - 627560
AN - SCOPUS:0018134771
VL - 253
SP - 1627
EP - 1631
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 5
ER -