The in vivo incorporation of mannose, retinol and mevalonic acid into phospholipids of hamster liver

Robert M. Barr; Luigi M. De Luca

doi:10.1016/0006-291X(74)90212-5

The in vivo incorporation of mannose, retinol and mevalonic acid into phospholipids of hamster liver

Robert M. Barr, Luigi M. De Luca

Research output: Contribution to journal › Article › peer-review

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Abstract

Hamsters were injected intraperitoneally with [¹⁴C]mannose, [¹⁴C]retinol and [³H]mevalonic acid. The livers were removed, extracted with chloroform-methanol and the lipids chromatographed on DEAE-cellulose and silicic acid. The hamster liver lipid contained a component which could be labelled with mannose and mevalonic acid. The properties of this compound were in accord with it being dolichyl-mannosyl-phosphate, a possible lipid intermediate required for the biosynthesis of some glycoproteins. [¹⁴C]Retinol and [¹⁴C] mannose were incorporated into another phospholipid which was labile to mild alkali conditions commonly used for the preparation of dolichyl-mannosyl-phosphate. The retinol labelled compound had similar properties to in vitro prepared mannosyl-retinyl-phosphate.

Original language	English (US)
Pages (from-to)	355-363
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	60
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(74)90212-5
State	Published - Sep 9 1974
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "The in vivo incorporation of mannose, retinol and mevalonic acid into phospholipids of hamster liver",

abstract = "Hamsters were injected intraperitoneally with [14C]mannose, [14C]retinol and [3H]mevalonic acid. The livers were removed, extracted with chloroform-methanol and the lipids chromatographed on DEAE-cellulose and silicic acid. The hamster liver lipid contained a component which could be labelled with mannose and mevalonic acid. The properties of this compound were in accord with it being dolichyl-mannosyl-phosphate, a possible lipid intermediate required for the biosynthesis of some glycoproteins. [14C]Retinol and [14C] mannose were incorporated into another phospholipid which was labile to mild alkali conditions commonly used for the preparation of dolichyl-mannosyl-phosphate. The retinol labelled compound had similar properties to in vitro prepared mannosyl-retinyl-phosphate.",

author = "Barr, {Robert M.} and {De Luca}, {Luigi M.}",

note = "Funding Information: Differentiation Control Section Lung Cancer Branch National Cancer Institute National Institutes of Health Bethesda, Maryland 20014",

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AU - Barr, Robert M.

AU - De Luca, Luigi M.

N1 - Funding Information: Differentiation Control Section Lung Cancer Branch National Cancer Institute National Institutes of Health Bethesda, Maryland 20014

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Y1 - 1974/9/9

N2 - Hamsters were injected intraperitoneally with [14C]mannose, [14C]retinol and [3H]mevalonic acid. The livers were removed, extracted with chloroform-methanol and the lipids chromatographed on DEAE-cellulose and silicic acid. The hamster liver lipid contained a component which could be labelled with mannose and mevalonic acid. The properties of this compound were in accord with it being dolichyl-mannosyl-phosphate, a possible lipid intermediate required for the biosynthesis of some glycoproteins. [14C]Retinol and [14C] mannose were incorporated into another phospholipid which was labile to mild alkali conditions commonly used for the preparation of dolichyl-mannosyl-phosphate. The retinol labelled compound had similar properties to in vitro prepared mannosyl-retinyl-phosphate.

AB - Hamsters were injected intraperitoneally with [14C]mannose, [14C]retinol and [3H]mevalonic acid. The livers were removed, extracted with chloroform-methanol and the lipids chromatographed on DEAE-cellulose and silicic acid. The hamster liver lipid contained a component which could be labelled with mannose and mevalonic acid. The properties of this compound were in accord with it being dolichyl-mannosyl-phosphate, a possible lipid intermediate required for the biosynthesis of some glycoproteins. [14C]Retinol and [14C] mannose were incorporated into another phospholipid which was labile to mild alkali conditions commonly used for the preparation of dolichyl-mannosyl-phosphate. The retinol labelled compound had similar properties to in vitro prepared mannosyl-retinyl-phosphate.

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The in vivo incorporation of mannose, retinol and mevalonic acid into phospholipids of hamster liver

Abstract

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