The in vivo incorporation of mannose, retinol and mevalonic acid into phospholipids of hamster liver

Robert M. Barr, Luigi M. De Luca

Research output: Contribution to journalArticlepeer-review

Abstract

Hamsters were injected intraperitoneally with [14C]mannose, [14C]retinol and [3H]mevalonic acid. The livers were removed, extracted with chloroform-methanol and the lipids chromatographed on DEAE-cellulose and silicic acid. The hamster liver lipid contained a component which could be labelled with mannose and mevalonic acid. The properties of this compound were in accord with it being dolichyl-mannosyl-phosphate, a possible lipid intermediate required for the biosynthesis of some glycoproteins. [14C]Retinol and [14C] mannose were incorporated into another phospholipid which was labile to mild alkali conditions commonly used for the preparation of dolichyl-mannosyl-phosphate. The retinol labelled compound had similar properties to in vitro prepared mannosyl-retinyl-phosphate.

Original languageEnglish (US)
Pages (from-to)355-363
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume60
Issue number1
DOIs
StatePublished - Sep 9 1974
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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