The impact of luteal phase support on gene expression of extracellular matrix protein and adhesion molecules in the human endometrium during the window of implantation following controlled ovarian stimulation with a GnRH antagonist protocol

Yulian Zhao, Jairo Garcia, Lisa Kolp, Christopher Cheadle, Annabelle Rodriguez-Oquendo, Nikos F. Vlahos

Research output: Contribution to journalArticle

Abstract

Objective: To evaluate the impact of two different luteal phase support protocols on gene expression of extracellular matrix (ECM) proteins and adhesion molecules in the human endometrium. Design: Eighty-four ECM protein and adhesion molecule genes were analyzed using array-based reverse-transcription polymerase chain reaction. Setting: Academic hospital. Patient(s): Nine oocyte donors. Intervention(s): Endometrial biopsies were obtained on the day of oocyte retrieval (group I) and 3-5 days later (group II) after randomization into 3 groups. Group IIa had no luteal phase support, group IIb had luteal support with micronized progesterone, and group IIc had luteal support with progesterone plus 17β-estradiol. Main Outcome Measure(s): Gene expression profiles in relation to different types of luteal phase support protocols. Result(s): Compared with the day of retrieval (group I), 24 genes were significantly up-regulated (4 in group IIa, 14 in group IIb, 22 in group IIc) and 14 genes were down-regulated (5 in group IIa, 2 in group IIb, 10 in group IIc) on day 3-5 (P

Original languageEnglish (US)
Pages (from-to)2264-2271
Number of pages8
JournalFertility and Sterility
Volume94
Issue number6
DOIs
StatePublished - Nov 2010

Fingerprint

Ovulation Induction
Extracellular Matrix Proteins
Luteal Phase
Endometrium
Gonadotropin-Releasing Hormone
Corpus Luteum
Gene Expression
Progesterone
Genes
Oocyte Retrieval
Self-Help Groups
Random Allocation
Transcriptome
Reverse Transcription
Oocytes
Estradiol
Outcome Assessment (Health Care)
Tissue Donors
Biopsy
Polymerase Chain Reaction

Keywords

  • adhesion molecules
  • extracellular matrix
  • implantation
  • luteal phase
  • microarray
  • Ovarian stimulation

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

@article{c0d84abf2b48406da8294b51f231fa1f,
title = "The impact of luteal phase support on gene expression of extracellular matrix protein and adhesion molecules in the human endometrium during the window of implantation following controlled ovarian stimulation with a GnRH antagonist protocol",
abstract = "Objective: To evaluate the impact of two different luteal phase support protocols on gene expression of extracellular matrix (ECM) proteins and adhesion molecules in the human endometrium. Design: Eighty-four ECM protein and adhesion molecule genes were analyzed using array-based reverse-transcription polymerase chain reaction. Setting: Academic hospital. Patient(s): Nine oocyte donors. Intervention(s): Endometrial biopsies were obtained on the day of oocyte retrieval (group I) and 3-5 days later (group II) after randomization into 3 groups. Group IIa had no luteal phase support, group IIb had luteal support with micronized progesterone, and group IIc had luteal support with progesterone plus 17β-estradiol. Main Outcome Measure(s): Gene expression profiles in relation to different types of luteal phase support protocols. Result(s): Compared with the day of retrieval (group I), 24 genes were significantly up-regulated (4 in group IIa, 14 in group IIb, 22 in group IIc) and 14 genes were down-regulated (5 in group IIa, 2 in group IIb, 10 in group IIc) on day 3-5 (P",
keywords = "adhesion molecules, extracellular matrix, implantation, luteal phase, microarray, Ovarian stimulation",
author = "Yulian Zhao and Jairo Garcia and Lisa Kolp and Christopher Cheadle and Annabelle Rodriguez-Oquendo and Vlahos, {Nikos F.}",
year = "2010",
month = "11",
doi = "10.1016/j.fertnstert.2010.01.068",
language = "English (US)",
volume = "94",
pages = "2264--2271",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - The impact of luteal phase support on gene expression of extracellular matrix protein and adhesion molecules in the human endometrium during the window of implantation following controlled ovarian stimulation with a GnRH antagonist protocol

AU - Zhao, Yulian

AU - Garcia, Jairo

AU - Kolp, Lisa

AU - Cheadle, Christopher

AU - Rodriguez-Oquendo, Annabelle

AU - Vlahos, Nikos F.

PY - 2010/11

Y1 - 2010/11

N2 - Objective: To evaluate the impact of two different luteal phase support protocols on gene expression of extracellular matrix (ECM) proteins and adhesion molecules in the human endometrium. Design: Eighty-four ECM protein and adhesion molecule genes were analyzed using array-based reverse-transcription polymerase chain reaction. Setting: Academic hospital. Patient(s): Nine oocyte donors. Intervention(s): Endometrial biopsies were obtained on the day of oocyte retrieval (group I) and 3-5 days later (group II) after randomization into 3 groups. Group IIa had no luteal phase support, group IIb had luteal support with micronized progesterone, and group IIc had luteal support with progesterone plus 17β-estradiol. Main Outcome Measure(s): Gene expression profiles in relation to different types of luteal phase support protocols. Result(s): Compared with the day of retrieval (group I), 24 genes were significantly up-regulated (4 in group IIa, 14 in group IIb, 22 in group IIc) and 14 genes were down-regulated (5 in group IIa, 2 in group IIb, 10 in group IIc) on day 3-5 (P

AB - Objective: To evaluate the impact of two different luteal phase support protocols on gene expression of extracellular matrix (ECM) proteins and adhesion molecules in the human endometrium. Design: Eighty-four ECM protein and adhesion molecule genes were analyzed using array-based reverse-transcription polymerase chain reaction. Setting: Academic hospital. Patient(s): Nine oocyte donors. Intervention(s): Endometrial biopsies were obtained on the day of oocyte retrieval (group I) and 3-5 days later (group II) after randomization into 3 groups. Group IIa had no luteal phase support, group IIb had luteal support with micronized progesterone, and group IIc had luteal support with progesterone plus 17β-estradiol. Main Outcome Measure(s): Gene expression profiles in relation to different types of luteal phase support protocols. Result(s): Compared with the day of retrieval (group I), 24 genes were significantly up-regulated (4 in group IIa, 14 in group IIb, 22 in group IIc) and 14 genes were down-regulated (5 in group IIa, 2 in group IIb, 10 in group IIc) on day 3-5 (P

KW - adhesion molecules

KW - extracellular matrix

KW - implantation

KW - luteal phase

KW - microarray

KW - Ovarian stimulation

UR - http://www.scopus.com/inward/record.url?scp=78049242909&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78049242909&partnerID=8YFLogxK

U2 - 10.1016/j.fertnstert.2010.01.068

DO - 10.1016/j.fertnstert.2010.01.068

M3 - Article

VL - 94

SP - 2264

EP - 2271

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 6

ER -