The immunohistochemical application of three anti-GFAB monoclonal antibodies to formalin-fixed, paraffin-embedded, normal and neoplastic brain tissues

Roger E. McLendon, Peter C. Burger, Charles N. Pegram, Lawrence F. Eng, Darell D. Bigner

Research output: Contribution to journalArticle

Abstract

The intermediate filament, glial fibrillary acidic protein (GFAP) has proven to be an important glial marker in diagnostic neuropathology. We report the histochemical application of three monoclonal antibodies (Mab) produced in this laboratory, 1B4, 2E1, and 4A11, which are monospecific to GFAP by radioimmunoassay, immunoblot electrophoresis, and immunoperoxidase histochemistry. The goal of this study was to compare the specificity and sensitivity of these Mab to GFAP on surgical brain biopsy specimens which had been routinely processed for diagnostic neuropathology with that of a high titer, highly specific, reference polyvalent anti-GFAP antiserum. The Mab stained astrocytes specifically in normal brain. When combined in a “cocktail” preparation, the quality of the immunoperoxidase detection of GFAP by these Mab closely approached that of the reference serum in 71 intracranial and intraspinal neoplasms. As these three Mab represent a continuous supply of a well defined, monospecific reagent, the monoclonal “cocktail” represents a standard reagent for large multi-institutional studies and for studies extending over a period of time.

Original languageEnglish (US)
Pages (from-to)692-703
Number of pages12
JournalJournal of neuropathology and experimental neurology
Volume45
Issue number6
DOIs
StatePublished - Nov 1986

Keywords

  • Astrocyte
  • Central nervous system
  • Formalin fixation
  • Glial fibrillary acidic protein
  • Intermediate filament
  • Monoclonal antibodies
  • Neoplasms
  • Trypsin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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