Abstract
In an estimate of the proliferative potential of nervous system neoplasms in 100 adult patients, the proliferating cells (G1, S, G2, and M phases) were demonstrated immunohistochemically by means of the monoclonal antibody Ki-67. The percentage of cycling cells was calculated in immunostained frozen sections by the peroxidase-antiperoxidase method. The histologic grade and known biologic behavior of gliomas were reflected in the percentages of stained cells, which ranged from 0.3% in a pilocytic astrocytoma to 17.2% in a glioblastoma multiforme. All three cases in which stained cells constituted over 20% were metastatic carcinoma. The percentage of stained cells in pituitary adenomas showed a wider range (0.2-3.7%) than that of the S-phase fraction obtained by Nagashima et al (0.1 -1.5%). These results indicate that Ki-67 staining is technically suitable as a routine method of estimating the proliferating cell fraction of nervous system neoplasms, and this information may be useful in predicting their biologic behavior.
Original language | English (US) |
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Pages (from-to) | 1147-1151 |
Number of pages | 5 |
Journal | Neurologia Medico-Chirurgica |
Volume | 27 |
Issue number | 12 |
DOIs | |
State | Published - 1987 |
Externally published | Yes |
Keywords
- Ki-67 monoclonal antibody
- neoplasms
- nervous system
- proliferating cells
ASJC Scopus subject areas
- Surgery
- Clinical Neurology