The Identification of Proliferating Cells in Nervous System Neoplasms by Use of the Monoclonal Antibody Ki-67

Taichiro Shibata, Peter C. Burger, Paul Kleihues

Research output: Contribution to journalArticlepeer-review

Abstract

In an estimate of the proliferative potential of nervous system neoplasms in 100 adult patients, the proliferating cells (G1, S, G2, and M phases) were demonstrated immunohistochemically by means of the monoclonal antibody Ki-67. The percentage of cycling cells was calculated in immunostained frozen sections by the peroxidase-antiperoxidase method. The histologic grade and known biologic behavior of gliomas were reflected in the percentages of stained cells, which ranged from 0.3% in a pilocytic astrocytoma to 17.2% in a glioblastoma multiforme. All three cases in which stained cells constituted over 20% were metastatic carcinoma. The percentage of stained cells in pituitary adenomas showed a wider range (0.2-3.7%) than that of the S-phase fraction obtained by Nagashima et al (0.1 -1.5%). These results indicate that Ki-67 staining is technically suitable as a routine method of estimating the proliferating cell fraction of nervous system neoplasms, and this information may be useful in predicting their biologic behavior.

Original languageEnglish (US)
Pages (from-to)1147-1151
Number of pages5
JournalNeurologia Medico-Chirurgica
Volume27
Issue number12
DOIs
StatePublished - 1987

Keywords

  • Ki-67 monoclonal antibody
  • neoplasms
  • nervous system
  • proliferating cells

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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