The identification and characterization of a GABAergic system in the cholinergic neuroblastoma x glioma hybrid clone NG108-15

Charles D. Searles, Harvey S. Singer

Research output: Contribution to journalArticle

Abstract

γ-Aminobutyric acid (GABA) and glutamic acid decarboxylase (GAD) activity were identified and characterized in the cholinergic neuronal cell clone NG108-15. GAD activity is similar to form A in rat brain by being temperature sensitive and independent of pyridoxal 5′-phosphate in the assay mixture. Most of the NG108-15 GAD activity is inactivated by 1 mM amino-oxyacetic acid. In contrast to NG108-15 choline acetyltransferase, GAD activity is not enhanced when cells are grown with dibutyryl cyclic AMP. The GAD activity shows a cell-density dependent increase that does not correlate with changing level of endogenous GABA. The identification of GABAergic properties in the NG108-15 clone enhances its use as a neuronal model for studies of differentiation and neurotoxicity of drugs or chemicals.

Original languageEnglish (US)
Pages (from-to)373-376
Number of pages4
JournalBrain research
Volume448
Issue number2
DOIs
StatePublished - May 17 1988

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Keywords

  • Differentiation
  • Glutamic acid decarboxylase
  • NG108-15
  • Neuronal cell clone
  • Neurotransmitter
  • Pyridoxal 5′-phosphate
  • γ-Aminobutyric acid

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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