Abstract
Animal studies with transplantable tumor cell lines suggested that the sensitivities of the in vitro tumor cultures to certain anticancer drugs agreed with the drug sensitivities of the same tumors in vivo. Soft agar cloning techniques have been established for human myeloma and ovarian cancer cells. Refinement of the techniques now permits cloning of most human malignancies. Drug sensitivity studies have been conducted measuring the reduction in the number of tumor colonies formed. Clinical trials showed that approximately one half of patients whose cells demonstrate in vitro sensitivity to a drug will obtain a clinical response to the same drug. Patients whose cells appear resistant to a certain drug in vitro rarely respond to the drug clinically. In vitro drug sensitivity testing may eventually allow some individualization of chemotherapy. In addition it already appears that these techniques may be excellent for screening new drugs with specific anticancer potentials.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 33-45 |
| Number of pages | 13 |
| Journal | Investigational New Drugs |
| Volume | 1 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 1983 |
| Externally published | Yes |
Fingerprint
Keywords
- clinical correlation
- development drug screening
- drug sensitivity testing
- soft agar cloning
- tumor stem cells
ASJC Scopus subject areas
- Pharmacology
- Molecular Medicine
Cite this
The human tumor cloning assay in cancer drug development - A review. / Agre, Peter C; Williams, Thomas E.
In: Investigational New Drugs, Vol. 1, No. 1, 03.1983, p. 33-45.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The human tumor cloning assay in cancer drug development - A review
AU - Agre, Peter C
AU - Williams, Thomas E.
PY - 1983/3
Y1 - 1983/3
N2 - Animal studies with transplantable tumor cell lines suggested that the sensitivities of the in vitro tumor cultures to certain anticancer drugs agreed with the drug sensitivities of the same tumors in vivo. Soft agar cloning techniques have been established for human myeloma and ovarian cancer cells. Refinement of the techniques now permits cloning of most human malignancies. Drug sensitivity studies have been conducted measuring the reduction in the number of tumor colonies formed. Clinical trials showed that approximately one half of patients whose cells demonstrate in vitro sensitivity to a drug will obtain a clinical response to the same drug. Patients whose cells appear resistant to a certain drug in vitro rarely respond to the drug clinically. In vitro drug sensitivity testing may eventually allow some individualization of chemotherapy. In addition it already appears that these techniques may be excellent for screening new drugs with specific anticancer potentials.
AB - Animal studies with transplantable tumor cell lines suggested that the sensitivities of the in vitro tumor cultures to certain anticancer drugs agreed with the drug sensitivities of the same tumors in vivo. Soft agar cloning techniques have been established for human myeloma and ovarian cancer cells. Refinement of the techniques now permits cloning of most human malignancies. Drug sensitivity studies have been conducted measuring the reduction in the number of tumor colonies formed. Clinical trials showed that approximately one half of patients whose cells demonstrate in vitro sensitivity to a drug will obtain a clinical response to the same drug. Patients whose cells appear resistant to a certain drug in vitro rarely respond to the drug clinically. In vitro drug sensitivity testing may eventually allow some individualization of chemotherapy. In addition it already appears that these techniques may be excellent for screening new drugs with specific anticancer potentials.
KW - clinical correlation
KW - development drug screening
KW - drug sensitivity testing
KW - soft agar cloning
KW - tumor stem cells
UR - http://www.scopus.com/inward/record.url?scp=0020918402&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020918402&partnerID=8YFLogxK
U2 - 10.1007/BF00180190
DO - 10.1007/BF00180190
M3 - Article
C2 - 6381376
AN - SCOPUS:0020918402
VL - 1
SP - 33
EP - 45
JO - Investigational New Drugs
JF - Investigational New Drugs
SN - 0167-6997
IS - 1
ER -